沙利度胺对溃疡性结肠炎疗效的实验研究.docVIP

沙利度胺对溃疡性结肠炎疗效的实验研究 　　【摘要】 目的 通过检测大鼠血清中肿瘤坏死因子-α（TNF-α）的浓度， 观察沙利度胺治疗由2， 4， 6-三硝基苯磺酸（TNBS）诱导的溃疡性结肠炎（UC）的疗效。方法 21只TNBS结肠炎动物模型， 随机分为模型组、柳氮磺吡啶组及沙利度胺组， 每组7只。模型组不给予干预、沙利度胺组给予沙利度胺200 mg/（kg?d）、柳氮磺吡啶组给予柳氮磺吡啶100 mg/（kg?d）， 测定大鼠血清中TNF-a的浓度， 探讨沙利度胺对UC的疗效。结果 沙利度胺组大鼠腹泻次数减少， 部分有隐血阳性， 一直未出现肉眼血便。沙利度胺组、柳氮磺吡啶组、模型组大鼠TNF-a的浓度分别为（19.51±8.06）、（40.88±14.24）、（115.32±9.60）pg/ml， 沙利度胺组降低血清TNF-α浓度的幅度高于柳氮磺吡啶组， 差异有统计学意义（P0.01）。结论 沙利度胺治疗UC有效， 能显著减轻结肠炎症， 降低TNF-α浓度强于柳氮磺吡啶。 　　【关键词】 沙利度胺；柳氮磺吡啶；2， 4， 6-三硝基苯磺酸；溃疡性结肠炎 　　DOI：10.14163/j.cnki.11-5547/r.2016.14.211 　　Experimental research of curative effect by thalidomide for ulcerative colitis XIA Ting-ting， XU Hong-yu. Department of Gastroenterology， Hulun Buir City People’s Hospital， Hulun Buir 021000， China 　　【Abstract】 Objective To observe curative effect by thalidomide for 2，4，6-trinitro-benzene-sulfonic acid （TNBS）-induced ulcerative colitis （UC） by detecting serum tumor necrosis factor-α （TNF-α） concentration in rats. Methods TNBS colitis animal model was made， and 21 rats were randomly divided into model group， sulfasalazine group and thalidomide group， with 7 rats in each group. No intervention was taken in model group. Thalidomide group received thalidomide by 200 mg/（kg?d）， and sulfasalazine group received sulfasalazine by 100 mg/（kg?d）. Detection was made on serum TNF-α concentration to investigate curative effect by thalidomide for UC. Results Thalidomide group had reduced diarrhea times， and some rats had positive occult blood， without any visible bloody stools. TNF-α concentrations in thalidomide group， sulfasalazine group and model group were respectively （19.51±8.06）， （40.88±14.24）， （115.32±9.60） pg/ml. Thalidomide group had higher reducing degree of serum TNF-α concentration than sulfasalazine group， and the difference had statistical significance （P0.01）. Conclusion Thalidomide is effective in treating UC， and it can remarkably relieve colitis. It shows better effect in reducing TNF-α concentration than sulfasalazine. 　　【Key wor