心复康口服液对心肌梗死后心衰大鼠心肌组织mit―CK mRNA及蛋白表达的影响.docVIP

心复康口服液对心肌梗死后心衰大鼠心肌组织mit―CK mRNA及蛋白表达的影响 　　摘要：目的 观察心复康口服液对心肌梗死心衰大鼠心肌组织线粒体肌酸激酶（mit -CK） mRNA及蛋白表达的影响。方法 采用结扎SD大鼠左冠状动脉前降支复制心肌梗死后心衰大鼠模型，用心复康口服液于术后24 h 灌胃给药至6周；以卡托普利作为阳性对照药，观察大鼠梗死周围心肌组织mit-CK mRNA及mit-CK蛋白表达水平。结果 第6周末，与模型组比较，卡托普利组、心复康口服液组心肌mit-CKmRNA、mit-CK蛋白表达均上调（P0.05）。结论 心复康口服液可上调心肌梗死后心衰大鼠心肌细胞mit-CKmRNA的表达，促进mit-CK蛋白含量增加，达到改善心肌细胞能量穿梭紊乱，重塑心肌梗死后心衰大鼠心肌能量代谢作用。 　　关键词：心力衰竭；心复康口服液；能量代谢；线粒体肌酸激酶；心肌梗死 　　中图分类号：R542.2 R256.2 文献标识码：A 　　doi：10.3969/j.issn.1672-1349.2014.04.041 　　文章编号：1672-1349（2014）04-0461-03 　　Effect of Xinfukang Oral Liquid on the Expression of mit-CK mRNA and Protein in Rats with Heart Failure after Myocardial Infarction 　　Du Bai，Hu Yuanhui，Ma Tiemin，et alGuang’anmen Hospital，China Academy of Chinese Medical Sciences（Beijing 100053） 　　Abstract：Objective To study the effect of Xinfukang oral liquid（XOL） on the expression of mitochondrialcreatine kinase（mit -CK） mRNA and protein in rats with heart failure after myocardial infarction.Methods The rat models of heart failure after myocardial infarction were created by ligating their left anterior descending coronary arteries.after 24 hours of creating models，rats in drug groups were fed on XOL and captopril through gastotube for 6 weeks，while captopril group was served as control.The expression of mit-CK mRNA and protein around infarction area were observed.Results Compared with mode group，the expression of mit-CK mRNA and protein in captopril group and XOL group were increased at 6th weekend （P0.05）.Conclusion XOL can effectively increase the expression level of mit-CK mRNA，as well as the protein content of mit-CK of rats with heart failure after myocardial infarction，and thus help to improve heart failure myocardial energy shuttle and remodel the process of myocardial energy metabolism function. 　　Key words： heart failure；Xinfukang oral liquid； energy metabolism；mit-CK；myocardial infarction 　　能量代谢障碍贯穿于心肌从代偿性肥大到衰竭的全过程，是心力衰竭（心衰）发生、发展和恶化的重要因素之一[1]。研究改善能量代谢的措施，对于防治心衰的发生发展具有重要意义。心复康口服液是临床治疗气虚血瘀型充血性心力衰竭的经验方