植物乳杆菌在马铃薯秧藤汁液中的适应性驯化培养研究.docVIP

植物乳杆菌在马铃薯秧藤汁液中的适应性驯化培养研究 　　摘要 将一株高产酸植物乳杆菌，在含有马铃薯秧藤汁液的一组MRS液体培养基中连续转移接种培养，组内5个培养基中的秧藤汁液浓度呈阶梯性递增状态，对完成连续驯化培养后的菌种进行冷冻干燥保存。进一步将该驯化菌和未驯化菌进行同步解冻激活后，分别接入含有马铃薯秧藤固体颗粒的液体培养基中培养48 h，结束后分离出各自培养基中的马铃薯秧藤固体颗粒部分和液体部分，采用比色法分别测定固体和液体部分中糖苷生物碱的含量；分别测定液体部分的pH值和总细菌细胞数。结果表明：在驯化菌的培养体系中，总细菌细胞数含量显著高于对照组；pH值显著低于对照组；马铃薯秧藤固体颗粒中糖苷生物碱含量明显低于对照组；培养液中的糖苷生物碱显著高于对照组。 　　关键词 马铃薯秧藤；植物乳杆菌；糖苷生物碱；青贮饲料 　　中图分类号 S816.5+3 文献标识码 A 文章编号 1007-5739（2015）14-0269-03 　　Study on the Cultivation of Lactobacillus of Adaptive Acclimation to the Juice of Potato Cane 　　ZHANG Xiong-jie 1 LU Peng-fei 2 CHEN Hao 3 LI Zhi-qiang 3 LIU Quan-guo 3 　　（1 Innner Mongolia Horizon Oasis Research Center of Characteristic Biologic Resource，Hohhot Innner Mongolia 010018； 　　2 Inner Mongolia Agriculture University； 3 Innner Mongolia Greed Seed industry of Potato Co.，Ltd） 　　Abstract In the experiment，the plant lactobacillus which have high acid production was transferred into a group of MRS liquid nutrient medium for the continuous transfer and cultivation，In this group of 5 liquid medium the juice of potato cane was the ladder of increasing state.Then，the freeze drying of the strains after continuous cultivation was carried out.Further，after thawing activation of the domesticated bacteria and not domesticated bacteria synchronously，They were inoculated to the liquid nutrient medium which included the juice of potato cane cultivate 48 h.After the training the potato cane solid particles and liquid parts in the medium was each separated.The contents of the Glycosides alkaloids in the solid and liquid parts were determined respectively by colorimetric method；pH values and total bacterial count were determined in the liquid parts.The results showed that in the cultivation system of the domesticated bacteria，the total bacterial cell number was significantly higher than that of the control group；pH value was significantly lower than that of the control group；Glycosides alkaloids content in the potato cane solid particles was significa