Propofolexertsitsanti-inflammatoryeffectinAlveolartypeIIepithelialcellsthroughdownreg.docVIP

Propofol exerts its anti-inflammatory effect in Alveolar type II epithelial cells through downregulation of CD14 and TLR4 expression Ling Ma, Weimin Chen, Xiuying Wu, Lingxin Meng, Yuji Fujino Authors: Ling Ma, M.D.1, Xiuying Wu, M.D, Ph.D2， Weimin Chen, M.D, Ph.D3, Yuji Fujino, M.D, Ph.D 4 Lecturer, Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China Associate Professor, Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China Professor, Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China Associate Professor, Department of Anesthesiology and Intensive Care Medicine, Osaka University Medical School, Osaka, Japan Corresponding author: Weimin Chen. Mailing address: Department of Anesthesiology, No.36 Sanhao Street, Heping District, Shenyang, 110004, China. Phone: +86 Email: chenwm@sj-hospital.org Running title: propofol and alveolar type II epithelial cell Summary Background: We investigated whether LPS induced inflammation in ATII is through CD14 and TLR4 and the effect of different dosage of propofol on the inflammation in primary cultured rat alveolar epithelial type II (ATII) cells. Methods: The cultured ATII cells were randomly assigned to one of the following five groups: Group C: AT II cells in untreated group (control) was cultured in the absence of propofol and LPS; Group LPS: treated with 1μg. -1ml LPS; Group P1: treated with 1μ.ml-1 LPS and 25μM propofol; Group P2: treated with 1μg.ml-1 LPS and 50μM propofol; Group P3: treated with 1μg.ml-1 LPS and 100μM propofol. ATII cells in untreated and propofol control groups were cultured at 37°C for 3 h. CD14 and TLR4 mRNA were detected using real-time PCR. Western blot were used to detect CD14 and TLR4 protein expression. CD14 and TLR4 expression on the AT II cells were imaged using immunofluorescence. TNF-α ?production were determined using ELISA kit. Results: LPS stimulation re