生物信息学实验四、蛋白质序列分析及结构预测讲解.doc

实验四、蛋白质序列分析及结构预测 用BioEdit等软件进行序列分析 打开FASTA格式序列 1、Sequence-Protein-Amino Acid Composition 分子质量；氨基酸组成 Protein: gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA Length = 248 amino acids Molecular Weight = 27462.22 Daltons Amino Acid Number Mol% Ala A 25 10.08 Cys C 9 3.63 Asp D 13 5.24 Glu E 14 5.65 Phe F 6 2.42 Gly G 10 4.03 His H 10 4.03 Ile I 3 1.21 Lys K 8 3.23 Leu L 37 14.92 Met M 5 2.02 Asn N 4 1.61 Pro P 18 7.26 Gln Q 10 4.03 Arg R 22 8.87 Ser S 15 6.05 Thr T 16 6.45 Val V 15 6.05 Trp W 4 1.61 Tyr Y 2 0.81 氨基酸组成表 helical wheel diagram Hydrophobic Moment matrix with Eisenberg consensus scale 疏水性 KyteDoolittle Mean Hydrophobicity Profile Eisenberg Scale Mean Hydrophobicity Profile Cornette Scale Mean Hydrophobicity Profile Parker HPLC Scale Mean Hydrophobicity Profile Boyko Scale Mean Hydrophilicity Profile Hopp%Woods Scale Mean Hydrophilicity ProtParam tool /protparam/ ProtParam?(References?/?Documentation) is a tool which allows the computation of various physical and chemical parameters for a given protein stored in?Swiss-Prot or TrEMBL?or for a user entered sequence. The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, estimated half-life, instability index, aliphatic index and grand average of hydropathicity (GRAVY) (Disclaimer). 输入FASTA格式序列 跨膜区分析 进入CBS 依次进入TMHMM Welcome to CBS http://www.cbs.dtu.dk/index.shtml CBS Prediction Servers http://www.cbs.dtu.dk/services/ TMHMM /protparam/ 输入FASTA格式序列 结果 Data 部分数据 # WEBSEQUENCE # AA inside membr outside 1 A 0.00271 0.00000 0.99729 2 T 0.00267 0.00004 0.99729 3 G 0.00265 0