慢性应激大鼠肝郁脾虚证水通道蛋白的表达讲解.docVIP

慢性应激大鼠肝郁脾虚证水通道蛋白的 表达 陈婷婷1，宋炜熙*12，曾光12，唐燕萍12，陈北阳3，张国民3 （1湖南中医药大学中西结合学院内儿科教研室，2分子病理实验室，3病理教研室 湖南 长沙 410208) [摘要] 目的 观测水通道蛋白在慢性应激肝郁脾虚证模型大鼠组织中的表达，研究逍遥丸对其表达的影响。方法 30只大鼠随机分为正常组、模型组及中药组，慢性应激抑郁模型加过度疲劳及饮食失节法复制大鼠肝郁脾虚证；中药组造模第7天始每天1次逍遥丸灌胃。以免疫组化研究AQP4及AQP3、AQP8在大鼠海马及胃肠中的表达。结果 模型组海马区AQP4下调，与正常组相比，差异有统计学意义（P0.05），3组平均光密度分别为0.25±0.03、0.21±0.06、0.23±0.07；中药组小肠黏膜中AQP3的表达上调，3组小肠黏膜黏膜的平均光密度分别为0.15±0.02、0.17±0.02、0.21±0.02，与正常组相比，差异有统计学意义(P0.05）；，AQP8在模型组肠黏膜及中药组胃、结肠粘膜中表达上调，差异有统计学意义（P0.05）。3组胃黏膜的平均光密度为0.15±0.01、0.16±0.01、0.16±0.01，小肠黏膜的平均光密度分别为0.19±0.04、0.21±0.04、0.22±0.04，结肠黏膜的平均光密度分别为0.22±0.06、0.19±0.01、0.21±0.04。结论：抑郁症肝郁脾虚模型大鼠AQPs的表达出现异常，逍遥丸能调节AQPs的表达。 关键词：AQPs；大鼠；肝郁脾虚证；逍遥丸；抑郁症 中图分类号：R ；R 文献标识码： The impact of Xiao Yao Wan on the expressions of aquaporins in Spleen Deficiency of chronic stress depressive rats* CHEN ting-ting; SONG wei-xi;ZENG guang; TANG yan-ping; CHEN bei-yang; ZHANG guo-ming (1.Hunan University of Traditional Chinese Medicine Institute of TCM diagnosis, Changsha,Hunan Province 410208, P.R.China; 2.Pediatric and Internal Department of Integrative Medicine College, 3.Molecular Pathology Laboratory, Hunan University of Traditional Chinese Medicine,Changsha,Hunan Province 410208, P.R.China) Abstract: 【Objective】 Observe aquaporins’ expressions in chronic stress liver stagnation and spleen deficiency model rats research the expressions of Xiao Yao Wan’s affection.【Methods】Liver stagnation and spleen deficiency model rats were replicated by chronic milled unpredicted stress and fatigue and diet disloyal. 30 rats were randomly divided into normal control group, model control group and Chinese intervention group, from the 7th day of making model, Chinese medicine group was intragastric administration Xiao Yao Wan one time everyday. AQP4 and AQP3, AQP8 expressions on hippocampus of rats model and gastrointestinal were detected by immunochemical staining method.【Results】The expressions of AQP4 of the model group was down-regulati