基于SOAR1过量表达植物对ABA高耐受性的除草研究.docVIP

基于SOAR1过量表达植物对ABA高耐受性的除草研究 摘要：为研究天然植物激素脱落酸（ABA）作为转基因植物选择性除草剂以实现环境友好型除草策略的可能性，采用改变拟南芥SOAR1基因表达的方法可以大幅度调控植物对ABA的耐受性，通过SOAR1基因过量表达获得对ABA耐受性高度增强的转基因植物。进一步通过植物在不同生长时期对ABA耐受性的试验检测ABA除草剂的有效浓度。结果表明，SOAR1过量表达转基因植物种子萌发和幼苗生长对ABA的耐受性超出了目前已知的极限，过量表达株系OE1、OE3和OE6所能够耐受ABA的最高浓度在种子萌发期为200 μM，在幼苗期生长期为500 μM。以最大限度抑制杂草生长发育和最少影响作物正常生长发育为前提，设定ABA除草剂的有效除草浓度范围在种子萌发期为3～100 μM，在幼苗生长期为10～200 μM。 关键词：脱落酸； SOAR1； ABA耐受性； 转基因植物； ABA除草剂 中图分类号：S451 文献标识码：A 文章编号：0439-8114（2016）05-1172-04 DOI：10.14088/ki.issn0439-8114.2016.05.021 Potential Weed Control Based on High ABA Tolerance of SOAR1-overexpression Plants JIANG Shang-chuan， MEI Chao， WANG Xiao-fang， ZHANG Da-peng （ School of Life Sciences， Tsinghua University， Beijing 100084，China ） Abstract： To study the potential eco-friendly weed control strategy with abscisic acid （ABA） as the selective herbicide for transgenic plants， we employed plant materials with changes in the expression level of SOAR1 which sharply regulate ABA tolerance in Arabidopsis. Transgenic plants with dramatically increased ABA tolerance can be obtained by overexpressing SOAR1. Additionally， a set of ABA tolerance assays during different growth stages were conducted to test the effective concentration of ABA’s herbicidal activity. The results showed that the ABA tolerance of SOAR1-overexpression plants goes beyond the known extremity， and the highest endurable concentration of ABA for overexpression lines OE1， OE3 and OE6 was 200 μM during seed germination and 500 μM during seedling growth， respectively. With the precondition of maximum growth repression of weed and minimum growth inhibition of transgenic plants， the effective concentration range of ABA’s herbicidal activity was 3～100 μM during seed germination and 10～200 μM during seedling growth， respectively. Key words： abscisic acid （ABA）； SOAR1； ABA tolerance； transgenic plant； ABA herbicide 天然植物激素脱落酸（Abscisic acid，ABA）参与调控植物生长发育各个阶段，包括种子休眠、萌发，幼苗生长、侧根生长、气孔运动以及营养生长向生殖生长转换等过程，并在植物对外界多种逆境胁迫的响应中起着重要作用[1-3]。 拟南芥中有450多个PPR（Pen