1云南古茶园遗传多样性的ISSR.docVIP

云南古茶树(园)遗传多样性的ISSR分析 季鹏章1,2,3，张俊2*，王平盛2，黄兴奇3*，许玫2，唐一春2，梁名志2 (1. 云南大学生命科学学院，云南 昆明，650091；2. 云南省农业科学院茶叶研究所，云南 勐海，666201；3. 云南省农业科学院生物技术与种质资源研究所，云南 昆明，650023） 摘要：云南省保存有20多万亩古茶园，这些古茶树（阿萨姆变种Camellia sinensis var. assamica）种质资源可能含有各种优良基因，对未来茶树良种选育有重要意义。本研究采用ISSR分子标记技术对云南省十个有代表性的古茶园遗传多样性进行分析，十个居群的多态位点百分率（P）为56.5%~90.9%；Nei’氏遗传距离（He）居群平均是0.281，阿萨姆变种水平内是0.461；Shannon多样性指数(Ho)居群平均是0.418，阿萨姆变种水平内是0.653。而居群间遗传分化系数Gst=0.391，和Shannon多样性指数分析（36.0%）和AMOVA分析结果（39.7%）相一致，说明阿萨姆变种60.9%的遗传变异来自居群內的个体间，39.1%的遗传变异来自居群间。研究结果揭示阿萨姆变种居群遗传多样性高，居群间遗传变异存在中度的遗传分化，这可能是由于茶树种内高度异交的特性和生境片段化所致。基于观察到的居群遗传信息，建议采取就地保护和迁地保护的保护策略。 关键词：茶树（Camellia sinensis var. assamica）；ISSR；遗传多样性；保护策略；古茶树 中图分类号：S571.1；Q523 文献标识码：A 文章编号：1000-369X（2007）04-271-9 Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR)Polymerase Chain Reaction JI Peng-zhang1,2,3, ZHANG Jun2*, WANG Ping-sheng2, HUANG Xing-qi3*, XU Mei2, TANG Yi-chun2, LIANG Ming-zhi2 (1. College of Life Sciences, Yunnan University, Kunming 650091, China; 2. Tea research institute, Yunnan Academy of Agricultural Sciences, Menghai, 666201, China; 3. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China) Abstact: Tea plant [Camellia sinensis var. assamica] populations in Yunnan province of China cultivated since ancient times are important genetic resources for future breeding of new tea cultivars. To evaluate the genetic diversity within and between Yunnan ten representative populations and to formulate conservation strategies for the ancient tea populations in Yunnan was conducted by ISSR technique in this study. The genetic diversity within populations calculated from Neis genetic diversity (HE) was estimated to be 0.281, on average, at the population level and 0.461 at the variety level, while Shannon indices (Ho) of population level and Assam variety level were 0.418 and 0.653, respectively and the percentage of polymorphic