蕺菜浸提液的抗氧化能力研究.docVIP

蕺菜浸提液的抗氧化能力研究 　　摘要：为了探究蕺菜（Houttuynia cordata Thunb.） 　　关键词：蕺菜（Houttuynia cordata Thunb.）浸提液；羟自由基；抗氧化；清除率 　　中图分类号：R282 文献标识码：A 文章编号：0439-8114（2014）04-0877-04 　　Antioxidation of Extracts from Houttuynia cordata Thunb. 　　ZHANG Cheng，KANG Man-ni，YE Hui-ying，SHI Ming-min，QI Xiao，LIU Qing-song，CHEN Chao 　　（Department of Bioengineering， School of Engineering Technology， Beijing Normal University Zhuhai Branch Campus， 　　Zhuhai 519087， Guangdong， China） 　　Abstract： To explore the extraction process and antioxidation of ethanol extracts from Houttuynia cordata Thunb.， the active components in Houttuynia cordata Thunb. were extracted by ethanol with certain concentration and its antioxidant ability were determined by spectrophotometry with phenanthroline-Fe2+. The hydroxyl radical（?OH） scavenging rates were used as test index. The major factors affecting extraction process were analyzed with single factor test. The extraction condition were optimized by L9（34） orthogonal tests and range analysis. The results showed that the optimal extraction conditions were extracting 2.0 h with the ratio（g：mL） of solid （Houttuynia cordata Thunb.） to solution of 1∶20 under 70 ℃. The maximum capacity of hydroxyl radical scavenging rate were 83.54%. It is indicated that the extractions from Houttuynia cordata Thunb. had a vast developing potential for its significant antioxidant ability. 　　Key words： extracts from Houttuynia cordata Thunb.； hydroxyl radical； antioxidation； scavenging rate 　　自由基是生物体生化反应的普遍介质。现代研究表明，许多疾病如心脑血管疾病、癌症以及人体衰老等过程都与自由基过剩有关[1]。机体内自由基的产生与消除是基本处于动态平衡的，但随着机体的衰老、抗氧化系统功能的下降，适当补充抗氧化剂有助于降低一些疾病的发生率，有利于机体健康。因而，如何开发利用抗氧化剂调节体内代谢、预防疾病、延缓衰老等已引起人们的广泛关注，成为现今各领域的研究热点[2，3]。且由于合成抗氧化剂存在一定的安全隐患，开发利用具有有效清除自由基效果的天然物质日益成为现代医药、保健领域的研究趋势。中国传统中草药是很有潜力的天然抗氧化剂资源，很多中草药的疗效与其抗氧化作用密切相关，从传统中草药中开发高效抗氧化剂不仅对食品工业，而且对医药、营养学均意义深远。近年来，一些研究指出厚朴、甘草、芡实、阿魏、红参、当归、金银花、银杏、五倍子、儿茶、虎杖等中草药及其成分均有抗氧化作用[4-8]。 　　蕺菜（Houttuynia cordata Thunb.）又名鱼腥草、侧耳根等，是三白草科（Saururaceae）蕺菜属（Houttuynia Thunb.）多年生草本植物，广泛分布在中国南方各省区