S1_MolecularIHCmethod.docVIP

S1_MolecularIHC method Molecular and immunohistochemistry method DNA extraction and quantification Formalin-fixed and paraffine-embedded (FFPE) tumor samples were sliced in 5 μm-thick sections and manually micro-dissected under light microscope control to isolate the highest amount of tumor cellularity compared to contaminating non-neoplastic cells (hopefully 70% or more). Samples were treated with xylene and 100% ethanol to remove all wax traces and DNA was then isolated using the GeneRead DNA FFPE kit (Qiagen, Hilden, Germany, , catalogue n. 180134) according to manufacturer’s instructio