杜仲饮片HPCE指纹图谱的(中药材)概览.docVIP

杜仲饮片HPCE指纹图谱的研究 摘要 目的建立饮片HPCE-DAD指纹图谱分析方法，并对及其炮制品的指纹谱进行比较。方法：采用毛细管电泳法进行色谱分离，以mmol·L –1硼砂-20 mmol·L –1 磷酸二氢钠-10%甲醇(pH= 10.0)为运行缓冲液，分离电压为20 kV，波长为210nm，以松脂醇二葡萄糖苷为参照物（IS），测定其指纹图谱，并作模糊聚类法分析和相似度评价。结果： 初步建立了以个共有峰为特征指纹信息的HPCE-DAD指纹图谱；发现少数饮片的指纹图谱有一定差异，生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论方法准确可靠，重现性好，可作为饮片内在质量评价的依据。关键词 ；二极管阵列检测法；指纹图谱Studies on HPCE-DAD fingerprint of Eucommiae Cortex FU Xing-sheng, HAN Le , LIU Xun-hong*, XU Hu , LI Jun-song , CAI Bao-chang，YANG Yan (Nanjing University of Chinese Medicine, Nangjing 210046, China) Abstract Objective: To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE-DAD and compare the fingerprints of Eucommiae Cortex and its processed products. Method: Based on the mode of high performance capillary electrophoresis，0mmol·L-1 sodium borate-20 mmol·L–1 sodium dihydrogen phosphate-10% methanol (pH10.0) was used as buffer solution. The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Pinoresinol diglucoside was used as a reference standard，the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. Result: -DAD fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Eucommiae Cortex and its processed products，there were obvious differences in the relative areas of common peaks. Conclusion: The method is reliable， accurate and can be used for quality control of . Key words Eucommiae Cortex；-DAD；Fingerprint.的干燥树皮，具有补肝肾，强筋骨，安胎等功效，用于肝肾不足、腰膝酸痛、筋骨无力、头晕目眩、妊娠漏血、胎动不安等症[1]。现代研究表明，松脂醇二葡萄糖苷、绿原酸和环烯醚萜这种7,8]。本文采用高效毛细管电泳法对杜仲饮片指纹图谱的构建进行了探讨，并与其炮制品进行比较分析，以期为规范杜仲饮片市场及确保药材内在质量的均一性和稳定性提供较全面的质量控制手段。 1 仪器与材料 G1600-AX高效毛细管电泳仪（美国Agilent公司），配惠普化学工作站、二极管阵列检测器（DAD）、自动进样器；Rotavapor R-3旋转蒸发仪（瑞士BUCHI公司）；pHS-3C型pH计（上海康仪仪器有限公司）；KQ-500E型超声波清洗器（昆山市超声仪器有限公司）。硼砂（南京化学试剂一厂，分析纯），氢氧化钠（