ExpressionofCD155PVRenhancesadhesionsignalingin.pptVIP

Expression of CD155/PVR enhances adhesion signaling in 9L cells. Kevin E. Sloan et al. Cancer Res 2005;65:10930-10937 ?2005 by American Association for Cancer Research Expression of CD155/PVR enhances adhesion signaling in 9L cells. Mock- or CD155-transfected 9L cells were challenged to adhere to vitronectin-coated or uncoated tissue culture dishes for 90 minutes. Cell lysates were prepared and analyzed by immunoblot, using phospho-specific antibodies to candidate signaling proteins. Representative immunoblots show increased phosphorylation of Src, FAK, paxillin, p130Cas, and Crk in CD155-expressing 9L cells adhering to vitronectin compared with control cells left . Phosphorylation of ERK1/2 and Akt was unaffected by CD155/PVR expression. β-Actin is shown as a loading control. In contrast, much smaller changes in the phosphorylation state of these signaling proteins due to CD155/PVR expression were observed in 9L cells adhering to uncoated tissue culture plastic, with the exception of paxillin right .