化工实验汇报分析.ppt

Reporter：LuMin No.座机电话号码056 The?extracting?technology?of?orange peel? flavonoids?was?studied??柑橘皮中总黄酮提取工艺研究 柑橘皮中总黄酮提取工艺研究 2 Research steps and methods 1 Research Background and purpose 3 The results of the research and discussion 柑橘皮中总黄酮提取工艺研究 中国是柑橘的重要原产地，但柑橘皮大多被作为废物丢弃，造成资源浪费而且污染环境。新鲜的柑橘皮中含有丰富的黄酮类物质成分，在现代医学发展中，黄酮具有的药理作用使其受到了广泛关注。因此对其黄酮类化合物的提取具有广阔的前景。 柑橘皮中总黄酮提取工艺研究 Research Purpose Different ethanol concentration,extraction time,proportion?of?materials?and?solvent and extraction temperature on the total content of flavonoids have an impact. Therefore this experiment by changing the various influence factorsin order to determine the best technology of extracting flavonoids.不同的乙醇浓度、提取时间、料液比、提取温度对提取液中总黄酮含量会产生影响。 为此本实验通过改变各个影响因素，以确定提取总黄酮的最佳工艺。 柑橘皮中总黄酮提取工艺研究 2 Research steps and methods 1 Research Background and purpose 3 The results of the research and discussion 柑橘皮中总黄酮提取工艺研究 实验 步骤及方法 分光 光 度法 精确配制好50ug/ml标准芦丁乙醇水溶液。精密吸取芦丁标准溶液0.0、1.0、2.0、4.5、6.0、8.0、10.0mL?,分别置于25mL?容量瓶中，分别取1ml到试管中各加5?%亚硝酸钠溶液0.3mL?摇匀,放置6min?,加10%硝酸铝溶液0.3mL?摇匀,放置6min?,加1mol?/L氢氧化钠溶液4mL?,再用60%乙醇溶液稀释至刻度,放置15min?后,分别在510nm?处测定其吸光度（以试剂空白做参比）?以吸光度A?为纵坐标,浓度c为横坐标,绘制标准曲线。A 0.0312C+0.0101, R2 0.9986 从图1可以看出黄酮浓度与吸光值有良好的线性关系 芦丁标准曲线绘制 图一 柑橘皮中总黄酮提取工艺研究 2 Research steps and methods 1 Research Background and purpose 3 The results of the research and discussion 柑橘皮中总黄酮提取工艺研究 NO.1 The influence of different concentration of ethanol on the extraction yield 不 同乙醇浓度对提取率的影响 Complete randomalized design（单因素实验） NO.2 The influence of differently extractive time on the extraction yield 不同提取时间对提取率的影响 柑橘皮中总黄酮提取工艺研究 柑橘皮中总黄酮提取工艺研究 NO3. The influence of differently feed’s scale on the extraction yield 不同料液比对提取率的影响 柑橘皮中总黄酮提取工艺研究 NO.4 The influence of differently extractive temperature on the extraction yield 不同提取温度对提取率的影响 柑橘皮中总黄酮提取工艺研究 the?extracting?technology?of?orangepeel? flavonoids?was?studied?and?the?results?showed?that?the?optimum?extraction?conditions?were?:ethanol?concertration?90%,proportion?of?materia