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跑台训练增强大鼠的学习记忆及其海马BDNF基因表达 徐 波1, 2，黄 涛2，季 浏1, 2 （1. “青少年健康评价与运动干预” 教育部重点实验室; 2. 华东师范大学体育与健康学院，上海，200241 ） 摘要 目的：探讨8周跑台训练对大鼠学习记忆能力及其海马内脑源性神经营养因子（BDNF）基因表达的影响。方法：将24只雄性SD大鼠分为对照组（C组，n 8）和训练组（T组，n 16），T组进行8周的跑台训练。通过Y迷宫实验，所有大鼠在第7周进行学习能力测试，在第8周进行记忆能力测试。第8周最后一次训练后，随机将T组分为T1组（n 8）和T2组（n 8）。T1组训练后即刻与C组同时断头处死，两组交叉进行，T2组在训练后24时断头处死，采用RT-PCR法检测各组大鼠海马内BDNF的基因表达水平。结果：Y迷宫测试表明T组大鼠的学习记忆优于C组；与C组相比，T1组和T2组大鼠海马中BDNF的基因表达水平均显著升高（p 0.01）。结论：8周跑台训练可增强大鼠的学习记忆能力，这与海马内BDNF基因表达的增加有关。 关键词： 跑台训练；大鼠；学习记忆；海马；脑源性神经营养因子 Treadmill training enhanced learning-memory and BDNF mRNA expression in hippocampus of rats XU Bo 1, 2, HUANG Tao 2, JI Liu 1, 2 （Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education; 2. College of Physical Education and Health, East China Normal University, shanghai, 200241）Abstract Objective: The purpose of this study is to explore the effects of treadmill training on learning-memory and BDNF mRNA expression in hippocampus of rats. Method: 24 male SD rats were assigned into two groups: control group C, n 8 , training group T, n 16 . The T group rats took part in treadmill training for 8 weeks. In 7th week, all of the rats were participated the learning test by Y maze, and in 8th week, all of the rats were participated the memory test by Y maze. After the last treadmill training in 8th week，T groups were assigned random into two groups：T1 group n 8 and T2 group n 8 ．The rats of the T1 group were decollated immediately after the last running alternatelycontrol group，and the rats of T2 group were decollated after 24 hours of the last running．The expression of BDNF mRNA were inspected by RT-PCR．Results: Y maze test show that the ability of learning and memory for T group rats were better than the C group rats. Comparing with C group, the expression of BDNF mRNA increased significantly in T1 and T2 group（p 0.01）. Conclusion: 8 weeks treadmill training can facility the learning process and improve the memory of rats, which may have close