蛋白质的分离纯化2重点.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Protocol development checklist Select techniques and conditions compatible with sample stability. Remove proteases at an early stage. Reduce volume in early steps. Use combinations of different separation principles. Start with high selectivity – increase efficiency. Use few steps. Limit sample handling between purification steps. The capture (C) step aims at concentrating the sample and removing the bulk of the contaminants. speed and load capacity The intermediate purification (iP) step(s) aims