酶切位点保护性碱基.docVIP

酶 寡核苷酸序列 切割率% 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0  90  90 0  90  90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 90  90  90 90  90  90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50  90  90 90  90  90 BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10  90  90 25  90  90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0  90  90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0  90 BstE II GGGT A/T ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATG