实验一 鸡新城血凝(HA)和血凝抑制(HI)抗体水平测定法.docVIP

实验一 鸡新城疫血凝（HA）和血凝抑制（HI）抗体水平测定法 1．本次实验的目的和要求 掌握鸡新城疫血凝（HA）和血凝抑制（HI）抗体水平测定法，以便检查疫苗使用效果和制定合理免疫程序。 2．实验内容或原理3．需用的仪器试剂4．实验步骤5．教学方式6．考核要求 本次实验的目的和要求 掌握有机磷中毒的诊断要点 熟悉有机磷中毒的简易检验方法。 了解有机磷中毒的解毒原理和治疗措施。 2．实验内容或原理3．需用的仪器试剂4．实验步骤）5．教学方式6．考核要求2．实验内容或原理3．需用的仪器试剂小白鼠4．实验步骤生化鉴定1．糖发酵试验取纯培养物分别接种葡萄糖、单奶糖、甘露醇、蔗糖、麦芽糖、乳糖、鼠李糖发酵管，37℃培养2～3d，观察结果。　　2．吲哚试验取纯培养物接种蛋白胨水，37℃培养2～3d，加入吲哚试剂，观察结果。　　3．硫化氢试验取纯培养物接种醋酸铅琼脂，37℃培养18～24h，观察结果。　　4．对紫乳作用的观察取纯培养物接种紫乳培养基，37℃培养2～3d，观察结果。巴氏杆菌生化试验 细菌名称 葡萄糖 乳糖 麦芽糖 甘露醇 蔗糖 单奶糖 鼠李糖 吲哚试验 H2S试验 紫乳作用 动力 巴氏杆菌 + - - ?+ + + - + + - - 注： + 糖发酵试验中表示产酸不产气、其它试验中表示阳性； - 阴性。 动物试验 　　1．取病料制成1:10乳剂，或用细菌的培养液。　　2．取0.2～0.5ml皮下注射小白鼠，经24～48h动物死亡。置解剖盘内剖检观察其败血症变化，同时取心血、肝、脾组织涂片，分别进行美蓝染色或瑞氏染色、革兰氏染色，镜检可见大量两极浓染球杆状的巴氏杆菌，革兰氏染色阴性。5．教学方式6．考核要求2．实验内容或原理3．需用的仪器试剂4．实验步骤5．教学方式6．考核要求 be carried out in time rust and antirust paint twice. While skeleton construction curtain wall fireproof, antisepsis, mine should be simultaneously, all skeletons complete after the required time and concealed work inspection, acceptance of concealed work qualified before the stone plaque installed. Stone in stone before they can use pollution prevention performance, radiation energy, water absorption properties and bending strength of various performance testing protective treatment and six stone two times, and minimize chromatic aberration, chromatic aberration under control mainly in the specification sheet and the stone material control, specification order form should try to distinguish between separate parts orders. Stone cutting must be carried out according to the same part of the same batch, different parts of different facades of stone panels applied when cutting different symbols of letters or Arabic numerals differentiates, in order to facilitate timely and accurately identify different stone when installing special mounting parts. Resolutely put an end to abuse with abuse. In addition to control and the local color, the other in the stone wall construction should be carried out before pub