公猪新鲜与冷冻精子之存活能力及粒线体鞘完整性之评估.docVIP

公豬新鮮與冷凍精子之存活能力及粒線體鞘完整性之評估 Assessment of Viability and Mitochondrial Membrane Sheath in Fresh and Frozen Boar Sperm 研究生: 劉志濬 Chih-Chun Liu 指導教授: 謝來安 Lai-An Hsieh [摘要]Semen qulity evalution is the most important thing before artificial insemination. The objective of this study was to compare the vital stain of trypan blue and fluorometric technique to assess different living/killed ratio sperm of viability and mitochondrial membran sheath and utilization of fluorometric technique to estimate frozen-thawed sperm and different stage of frozen-thawed sperm viability. The parameters of motility, via