多药耐药基因转染对荷瘤小鼠骨髓造血细胞的保护作用 张秀亚，王 珊.doc

多药耐药基因转染对荷瘤小鼠骨髓造血细胞的保护作用 张秀亚，王 珊，李 圆，孔祥如，王江波(重庆医科大学附属儿童医院肿瘤外科，重庆400014) 摘要：目的 探讨MDR1基因转染荷瘤小鼠骨髓单个核细胞（BM-MNCs）后在体内的分布和对骨髓造血细胞的作用。方法 将32只H22肝癌荷瘤BALB/c小鼠随机分为4组：A正常对照组（不照射不回输），B空白对照组(照射并回输生理盐水)，C阴性对照组(照射并回输未转染的BM-MNCs)，D转染实验组(照射并回输已转染的BM-MNCs），每组8只。阿霉素超剂量化疗，监测化疗过程中各组外周血细胞和肿瘤体积的变化，用RT-PCR法和免疫组化法检测外源性人MDR1基因和P-gp在荷瘤小鼠体内的表达和分布。结果 化疗后各组红细胞和血小板均无明显变化，差异无统计学意义（P0.05）；D组白细胞数于化疗后第 4周降至（3.32±0.26），与化疗前（6.64±0.39）相比减少有统计学意义（P0.05），化疗后第3周起D组白细胞数高于C组，增高有统计学意义（P0.05））(；肿瘤 中图分类号：R735.7 中图法分类号：A The effection of haematogenesis in bone marrow after MDR1 gene transfection in tumor-bearing mice ZHANG Xiu-Ya, WANG Shan, LI Yuan, KONG Xiang-Ru, WANG Jiang-Bo（Department of Surgical Oncology, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China） Abstract: Objective To observe the foreign multidrug resistance gene-1 (MDR1) transfer to protect in gene therapy. Method Thirty－two of BALB／ tumor-bearing mices divide into four groups: nomal control（A）,blank (B), nagtive control(C) and tansfective control(D),each group have eight mices.variation；detect the distribution and expression of exogenous human MDR1 and P-gp by RT-PCR and immunohistochemistry. Result platelet count have no difference after chemotherapy(P0.05).The white cell count of D group decreased to (3.32±0.26) after 4w chemotherapy, there was difference compared with pro- chem- otheropy (6.64±0.39)(P0.05).The white cell count of D group was higher than the C group after 3w chemotherapy(P0.05). Tumor growth slower in the group treat with MDR1 transfer(P0.05).The MDR1 and P-gp expression was detected in bone marrow and PB mononuclear cells by RT-PCR and immunohistochemistry, there was no MDR1 and P-gp expression in heart,liver,lung, spleen and kidney.there was P-gp not MDR1 expression on tumor. Conclusion The transfer of MDR1 gene into tumor-bearing mice BM-MNCs may provide some degree of chemoprotection for BM haemopoiesis. there was no MDR1 and P-gp expression in heart, liver, lung, spleen and kidney. Key