姜黄素对髓母细胞瘤PI3KAKT通路的作用机制 .docVIP

姜黄素对髓母细胞瘤PI3K/Akt信号通路的作用 毛 蜀，何密斯，刘桂元，张 雄，李 昱， 唐 俐（400016 重庆，重庆医科大学病理生理学教研室，重庆医科大学病理学教研室，重庆医科大学神经科学研究中心） [摘要]目的 髓母细胞瘤PI3K/Akt信号通路20～100 μmol/L24、48、72 h设对照组（Ct组）、姜黄素处理组（Cur组）MTT法检测姜黄素对髓母细胞瘤Daoy细胞的生长抑制作用流式细胞术分析Daoy细胞凋亡率免疫细胞化学染色检测PI3K、p-Akt和Akt在细胞中表达情况；Western blot和RT-PCR分别检测PI3K、p-Akt和Akt蛋白及mRNA的表达水平。 不同浓度姜黄素作用不同时间后，细胞受到不同程度的抑制，呈时间-剂量依赖关系（P 0.05）48 h时抑制作用显著差异有统计学意义（F＝131.829，P＜0.05）IC50为35 μmol/L细胞凋亡。PI3K、Akt和p-Akt蛋白在中的阳性表达率分别为80.7、84.8和87.5阳性表达率明显下降，分别为25.3、58.8和26.7%。两组比较，差异有统计学意义（P 0.05）。Western blotPI3K、Akt和p-Akt蛋白1.141±0.032，1.047±0.174，1.173±0.013；Cur组PI3K和p-Akt蛋白降低，0.875±0.029，0.958±0.150。两组比较差异有统计学意义（t 15.530，33.482，P 0.05）RT-PCR显示发现PI3K、Akt mRNA±0.031，1.072±0.007；Cur组PI3K mRNA灰度值为0.833±0.033。两组比较差异有统计学意义（t 12.468，P 0.05）。 姜黄素通过抑制PI3k/Akt信号通路髓母细胞瘤细胞。 [关键词] 髓母细胞瘤；姜黄素；PI3K/Akt信号通路 [中图法分类号]　R739．41 　　［文献标志码］　A he role of Curcumin to PI3k/ Akt signaling pathway in human medulloblastoma Mao Shu，He Misi，Liu Guiyuan，Zhang Xiong，Li Yu，Tang Li （Department of Pathophysiology ， Department of Pathology ， Institute of Neuroscience， Chongqing Medical University，Chongqing，400016，China） [Abstract] Objective To investigate the role of Curcumin to PI3k/ Akt signaling pathway in human medulloblastoma.Methods Daoy cells were treated with curcumin at different concentrations 20～100 μmol/L and time intervals 24,48,72 hours when they were in the logarithmic growth phase, and then the Daoy cells were divided into the control group and the curcumin group. The proliferation and apoptosis of Daoy cells was analyzed by MTT assay and flow cytometry,respectively, and the expression of PI3K，Akt and p-Akt at mRNA and protein levels was detected by immunocytochemistry， RT-PCR and western blotting, respectively. Results Exposure of cells to curcumin resulted in decrease of cell proliferation rat in dose- and time-dependent manners P 0.05 ．The inhibition rates reached peak and a significant increase of cell apoptosis was observed when the concentration of curcumin was 35μmol/L and