Breeding of Phytohpthora Resistance Pepper Line Using Recurrent Selection and MAS.ppt

Recurrent Selection Methods Developed to systematically improve maize germplasm “Recurrent” refers to the repetition of the similar procedures in successive cycles of selection Goal is to improve traits inherited in a quantitative manner Rapid Recovery of Recurrent Genome via MAS * Breeding of Phytohpthora Resistance Pepper Line Using Recurrent Selection and MAS Horticultural Crops Breeding Lab. March 29, 2004 Lee, Wonphil Contents I. Phytophthora capsici??? II. Recent Advance in Phytophthora Resistance Pepper Line Breeding - Recurrent Selection - Comparative mapping III. Conclusion Phytophthora capsici - Oomycete causing pepper root rot, crown rot, foliar blight - Water saturated soil, warm soil temperature, heavy rainfall and irrigation (Bosland et al. 1999) Resistance Accessions Screening 1980 1990 2000 Line 29, PI201232, PI201234, AC311, Vania AC2258, CM331, Perennial, CM334 (Alcantara and Bosland, 1994; Bartual et al.,1994; Bosland and Lindsey,1991; Gil Ortega et al.,1991; Guerrero-Moreno and Laborde, 1980; Palloix et al.,1988) Genetic Analysis Qualitative Trait One or Two Dominant/Recessive (~Mid of 90’s) - Simple inheritance mode - Simple breeding scheme - Nevertheless NO AVAILABLE Phytophthora Resistance Pepper Line Quantitative Trait Polygenic Resistance Trait. Continuous Variation in Segregation Popl. Environmental Effect VP = VG + VE + VGxE - Complex Inheritance Mode - Comple Breeding Scheme History QTL mapping MAS Breeding resistance Line Comparative Mapping The backcross strategy proved to be efficient to rapidly recover an elite genetic background but is unsuitable when too many genes have to be transferred from the donor to the recipient parent. (Palloix et al., 2004) Disparities may have been caused by inconsistency in experimental designs, or by different genetic backgrounds or the susceptible parents. (Bosland et al., 1999) Recent Advance in Phytophthora Resistance Pepper Line Breeding Recent