第六章 质谱—6.8 联用技术.ppt

Why MS UV---Light absorbtion Fluorescence Detection---Photon absorption and emission Electrochemical detection---Oxidation/Reduction Refractive Index Detection---Bending a beam of light MS---It is based on a different physical property-m/z MS Values for Chromatographers 1. Molecular weight, and relative isotope ratio for identified compounds 2. Solve co-eluting compounds even after HPLC column separation 3. Confirm peak shift, small peak and unexpected one 4. CID for unknown compound analysis 5. Perform selected Ion Monitoring by on-line HPLC/ESIMS 6. APCI and ESI are complement for analysis M