第十章 糖类合成代谢.pptVIP

What are the major stages for CO2 assimilation via the Calvin cycle? Fixation stage: CO2 is condensed to a five-carbon acceptor (ribulose-1,5-bisphosphate) to form 3-phosphoglycerate; Reduction stage: 3-phosphoglycerate is reduced to glyceraldehyde-3-phosphate; Regeneration stage: ribulose-1,5-bisphosphate is regenerated using glyceraldehyde-3-phosphate. How was the “path of carbon” in photosynthesis revealed by Calvin? Mainly two methods were used: isotopic tracing and chromatographic separations of labeled intermediates; Inject 14CO2 into a algae suspension, then illuminate for a short per