类风湿五项在诊类风湿性关节炎中的应用,修改后.docVIP

AKA、CCP、类风湿因子在诊断类风湿性关节炎中的应用 邹薇薇，李欣 （新疆库尔勒市巴州人民医院医学检验科，新疆库尔勒 841000） 【摘要】 目的 探讨抗角蛋白抗体AKA、抗环瓜氨酸肽抗体CCP、类风湿因子IgA、IgG、IgM抗体RF-IgA、RF-IgG、RF-IgM在临床诊断自身免疫性类风湿性关节炎（RA）中的临床意义。方法 收集2009年4月~2010年1月新疆库尔勒市巴州人民医院门诊就诊患者的370份血清标本，采用间接免疫荧光法（IIF）和酶联免疫吸附法（ELISA）检测AKA、CCP、RF-IgA、RF-IgG、RF-IgM水平 。选取符合临床RA诊断患者225例，对照组113例分析AKA、CCP、RF-IgA、RF-IgG、RF-IgM在不同性别、年龄RA患者中的分布情况。 结果 在225份检测标本中，AKA、CCP、RF-IgA、RF-IgG、RF-IgM的阳性率分别为15.1%、20.9%、31.6%、29.3%、30.7%，男女比例分别为1:2.8、1:3、1:2.9、1:3、1:2.5。男女比例二者差异有统计学意义（x2=113.76，PO.O1）。AKA、CCP、RF-IgA、RF-IgG、RF-IgM的不同年龄组间阳性率差异也有统计学意义（≤25岁组0.4%、0.9%、%、1.3%、0.0%、2.2%，26～50组6.7%、8.0%、12.4%、12.0%、13.8% ，≥51岁组9.3%、12.0%、17.8%、17.3%、21.8%,x2=108.9 ，P均O.O1）。结论 临床RA就诊患者中AK、CCP、RF-IgA、RF-IgG、RF-IgM的阳性率在不同性别、年龄组间存在差异。 【关键词】 抗角蛋白抗体AKA，抗环瓜氨酸肽抗体CCP，类风湿因子IgA、IgG、IgM抗体；类风湿性关节炎；荧光抗体技术，酶联免疫吸附 Application of AKA,CCP,RF in diaqnosticate of rheumatism arthritis ZOU Wei wei ,LI Xin Department of Medical Laboratory, People Hospital of Bayinguoleng,Xinjiang 841000 ,China 【Abstract】 Objective To explore the positive distribution trend of general examine of Rheumatism in Rheumatism Arthritis(RA) and its significance in patients with RA . Methods The AKA,CCP,IgG,IgM,IgA in 370 specimens were measured by indirect immunofluorescence(IIF) and enzyme linked immnosorbent assay(ELISA) form department of clinical laboratory, People Hospital of Bazhou of Xinjiang form April of 2009 to January of 2010 .The 225 cases with RA patients were chosen. Meanwhile the control group was consist of 113 cases . The positive distribution of AKA、CCP、RF-IgA、RF-IgG、RF-IgM in gender groups ,age groups (≤25 years old ，26～50 years old ，≥51 years old) and the values of the results for RA patients diagnosis were evaluated. Results Of the 225 specimens, the positive rate of AKA , CCP , RF-IgA ,RF-IgG , RF-IgM was 15.1%,20.9%,31.6%,29.3%,30.7%,the positive rates for male patients and female patients were 1∶2.8,1:3,1:2.9,1:3,1:2.5。The positive rate in female patients was statistically higher than in m