Plackett-Burman联用Box-Behnken效应面优化裂褶菌多糖的提取工艺.docVIP

Plackett-Burman联用Box-Behnken效应面优化裂褶菌多糖的提取工艺 摘 要 本研究是用裂褶菌作为材料来进行裂褶菌多糖提取的，并采用响应面分析（RSA）的方法来对裂褶菌多糖的提取条件进行了初步的优化。首先运用Plackett-Burman试验设计法对影响裂褶菌多糖得率的四个因素（温度、时间、pH值、液料比）进行评价，从中筛选出来三个主要影响因素: 温度、时间、pH值。然后将这三因素应用Box-Behnken试验设计分析后得出裂褶菌多糖提取条件的最优组合，并且采用了三因素三水平的响应面分析方法，根据数据分析方法中的回归分析确定了各个因素与裂褶菌多糖得率之间的关系，并以多糖得率作为响应值做响应面曲面图和等高线图。结果显示为：温度为84 ℃，时间为1.7 h，pH值为10.6。得到的裂褶菌多糖得率为6.18 %，与理论上的裂褶菌多糖得率（6.3 %）相当，并且与单因素试验中的最高多糖得率（5.9 %）相比提高了，表明Plackett-Burman实验设计结合Box-Behnken试验设计分析法可以比较好的对裂褶菌多糖提取工艺进行优化。 关键词 裂褶菌多糖；提取；Plackett-Burman试验设计；Box-Behnken试验设计；响应面优化。 Optimization Extraction of Schizophyllan from Schizophyllum Commune Based on Plackett-burman Combined with Box-behnken response Surface Methodology Abstract This study was used as a material Schizophyllum to schizophyllan extracted and response surface analysis (RSA) method for the extraction conditions schizophyllan conducted a preliminary optimization. Packett-Burman design first used on the factors affecting the yield schizophyllan be evaluated, selected three main factors: temperature, time, pH value. Then these three factors were Box-Behnken experimental design analysis obtained after extraction conditions schizophyllan optimal combination, and uses three factors and three levels of response surface methodology, based on regression analysis identified factors and Schizophyllum Relations between polysaccharide extraction rate and yield response to polysaccharide made surface plot and response surface contour plots. The results show as follows: temperature was 46.358 ℃, time was 4.532 h, pH value of 5.523. The resulting schizophyllan yield 0.324, and the theoretical yield schizophyllan (0.328) fairly and with the highest single-factor test polysaccharide yield (0.223) compared to the increase, indicating that Packett-Burman design Combined with the response surface methodology can be good for schizophyllan extraction process optimization. Key words Schizophyllan; Extraction; Plackett-Burman desi