玉米芯诱导里氏木霉合成纤维素酶毕业论文.doc

SHANDONGＵＮＩＶＥＲＳＩＴＹ　ＯＦ　ＴＥＣＨＮＯＬＯＧＹ 毕 业 论 文 玉米芯诱导里氏木霉合成纤维素酶 学 院： 专业班级： 学生姓名： 学 号： 指导教师： 201年 月 摘要 纤维素类物质是自然界中最具潜力的一种可再生资源，其有效利用是目前人们关注的热点。作为降解纤维素的多组分酶系，纤维素酶降解纤维素特异性高，反应条件温和，环境污染小。但由于其组分复杂，合成代谢网络研究尚不清楚。此外，真菌纤维素酶多数是诱导酶，其合成须在诱导物存在下才能进行，通过添加诱导物及对纤维素酶合成途径进行调控是提高纤维素酶活性的有效方法。因此，研究以天然纤维素物质为底物的纤维素酶合成过程机理，充分挖掘现有生产菌株产纤维素酶的潜力，是解决纤维素酶诱导合成问题的关键。 本文对天然纤维素物质玉米芯诱导里氏木霉产纤维素酶的过程进行了分析，通过测定玉米芯酶解液中糖的组分，并以所测定到的组分为诱导物进行诱导产酶，发现除纤维素成分外，半纤维素水解产物半乳糖对纤维素酶的合成有诱导作用。通过优化半乳糖的诱导浓度，发现5g/L的半乳糖对纤维素酶的诱导最好。 关键词：里氏木霉、纤维素酶、玉米芯、诱导 Abstract Cellulose is a natural substance in the most potential as a renewable resource, and its efficient use is currently the focus of attention. As a multi-component enzymes degrade cellulose, cellulose degradation of cellulose high specificity, mild reaction conditions, environmental pollution. However, due to the complexity of its components, anabolic Network Research unclear. In addition, most of the fungal cellulase enzyme induction, induction of the synthesis must be carried out under the presence, and by adding the inducer of cellulase synthesis pathway to improve the cellulase activity regulating effective way. Therefore, the study of natural cellulosic materials as substrates cellulase synthesis mechanism to fully exploit the existing strains producing cellulase production potential is to solve the problem of liquid fermentation of cellulase key. In this paper, natural cellulose substances corncob induced Trichoderma reesei cellulase production process was analyzed by measuring the sugar in corn cob hydrolyzate components, and the group to which the measured inducer for induction into production enzymes, found that in addition cellulose ingredients, hemicellulose hydrolyzate galactose on cellulase synthesis induced. By optimizing the concentration of galactose induction, found 5g / L of galactose induction of cellulase best. Key words: Trichoderma reesei,