斑葉露兜树茎腐病病原菌的鉴定及其在植株体内的分布.docVIP

PAGE PAGE 1 建议： 1、前言说明研究的重要性，，，，前人有研究吗，知道是什么菌，提高菌鉴定意义的说明。 2、讨论中，要把研究的意义说出来，比如，明确了菌源，，未见报道，，，为防治提供依据，，， 3、我给你查了世界文献，不多，你看了后，说明几个问题，叶露兜树茎腐病有人鉴定过吗？Fusarium verticillioides 还可以发生在什么上面？前人都对这个研究作了什么？我们的研究要干什么。 4、题目“斑叶露兜树茎腐病的发生特性及其病原菌分子鉴定”  斑叶露兜树茎腐病病原菌的鉴定及其在植株体内的分布? ?收稿日期： 基金项目：国家863计划项目(2006AA10A211) *通讯作者Author for correspondence（E-mail：fzliubo@163.com） 肖荣凤，林抗美，郑雪芳，蓝江林，刘 波* （福建省农业科学院农业生物资源研究所，福建，福州 350003） 摘要：本研究分离与鉴定了引起斑叶露兜树茎腐病的病原菌并分析了病菌在该植株体内的分布特性。本研究采用常规病原菌分离方法、Booth镰刀菌鉴定方法及菌株的rDNA-ITS序列分析，确定从福建建新采集的斑叶露兜树茎腐病的病原菌为串珠镰刀菌明确了从福建建新采集的斑叶露兜树茎腐病的病原菌为串珠镰刀菌(Fusarium verticillioidesverticillioides)。，菌株的核苷酸序列在GENBANK中的登录号为EU717682。串珠镰刀该菌在斑叶露兜树植株体内的数量分布的研究结果表明，含菌量因植株部位和病情不同而有明显差异。就分离的部位而言，茎部的含菌量最大，其次为根部，而叶部的含菌量最少；就病情而言，病株的含菌量是无症株的23倍。。 关键词：斑叶露兜树；串珠镰刀菌； ITS序列分析；体内分布 中图分类号： 文献标识码：A 文章编号： Identification of Fusarium verticillioides causing root rot of Veitchs Screw Pine and distribution of the pathogens inside the plants XIAO Rong-feng, Lin Kang-mei, Zheng Xue-fang, LAN Jiang-lin, and LIU Bo (Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China) Abstract: The objectives of this study were to isolate and identify pathogen which caused root rot on the plant of Pandanus veitchii Dall, and to determine the distributions of this isolate inside the plant.caused the symptoms: vascular wilt, yellows, root rot, and damping-off on Pandanus veitchii Hort. The result showed that the pathogen was Fusarium verticillioides which was isolated from the plant of P.veitchii , in Jianxin, Fujian province, PR China, and identified by using Booth’s method and the sequence of its rDNA-ITS. The resulted showed that the pathogen was identified as F. moniliforme已改学名. GenBank accession number for the isolation nucleotide sequence was EU717682. Meanwhile, the distributions of F. verticillioidesthe pathogens were detected inside the symptomatic and asymptomatic P. veitchii plants to and find found that the amount of the pathogen was higher i