英文----经漆酶介体系处理后黄麻纤维中木质素结构的影响.doc

Structural changes of lignin in the jute fiber treated by laccase and mediator system Yongbing Zhang, Qiang Wang*, Xuerong Fan, Jiugang Yuan Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, PR China Abstract To study the structural changes of lignin in the jute fiber treated with laccase and mediator system (LMS), lignins from the control and LMS-treated jute fiber were isolated and characterized by gel permeation chromatography (GPC), elemental analysis, measurement of phenolic hydroxyl group content, FTIR and 1H NMR. The results showed that the molecular weights of the lignin from LMS-treated jute fiber were lower than those of the lignin from the control jute fiber. The contents of phenolic hydroxyl group, aliphatic hydroxyl group and methoxy group of the lignin from LMS-treated jute fiber decreased, while the content of carboxyl group increased. Keywords: laccase, jute fiber, lignin, degradation, mediator 1. Introduction The jute fiber lignin is composed of p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) units with a H/G/S composition of 2:32:66 and a S/G ration of 2.1 [1]. The lignin content in jute fiber is up to ca. 16%, which resulted in the coarseness and rigidity of the fiber. Therefore, jute fibers are mainly used to make the low-grade goods such as package fabrics and bags [2-3]. The removal of lignin from jute fiber is proved to be a key step in the manufacturing of high-value textile products. In the traditional process, the lignins in jute fibers are eliminated mainly in degumming using some chemical products, which often cause severe environmental pollution. In order to overcome the disadvantages of chemical degumming, enzymatic degumming has been attracted a great deal of attention. Laccases (benzenediol:oxygen oxidoreductases, EC ) are a widespread group of multi-copper enzymes [4]. It has been reported by many researchers that laccase can degrade or polymerize the phenoli