葡萄糖-6-磷酸脱氢酶对皮层注射FeCl 3 所致早期癫痫的保护作用.docVIP

葡萄糖-6-磷酸脱氢酶对皮层注射FeCl3所致早期癫痫的保护作用 蒋国辉，方 升，石全红，但 炜，詹 彦，崔荣周，王 佳，谢延风（400016 重庆，重庆医科大学附属第一医院神经外科） [摘要] 目的 探讨葡萄糖-6-磷酸脱氢酶（glucose-6-phosphate dehydrogenase，G6PD）在外伤后早期癫痫中的脑保护作用。方法 采用大脑皮质微量注射FeCl3溶液建立癫痫大鼠动物模型。将24只SD大鼠抽签随机分为对照组、实验组、干预组（n=8）G6PD激动剂1,25-(OH)2D3 1μg/(kg﹒d)，连续7d后，对照组为皮层注射pH为1.5的生理盐水5μl，实验组和干预组皮层注射100 mmol/L FeCl3 5μl。所有大鼠皮层注射后行脑电监测2 h，行为学观察6 h后处死；利用RT-PCR、Western blotG6PD表达，比色法检测大脑皮层脂质过氧化的主要代谢产物丙二醛（malondialdehyde，MDA）glutathione，GSHP0.05)；干预组皮层G6PD mRNA及蛋白表达水平均高于实验组（P0.05），但仍低于对照组（P＞0.05G6PD活性、GSH含量增高（P0.05)，MDA的含量显著降低（P0.0)。结论G6PD可提高大鼠脑皮层的抗氧化能力，对FeCl3所致的早期癫痫有一定的保护作用。 [关键字] 早期癫痫；葡萄糖-6-磷酸脱氢酶；还原型谷胱甘肽；丙二醛 Glucose-6-phosphate dehydrogenase protects early epilepsy in rats after intracortical injection of ferric chloride Jiang Guohui, Fang Sheng, Shi Quanhong, Dan Wei, Zhan Yan, Cui Rongzhou, Wang Jia, Xie Yanfeng (Department of Neurosurgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016,China) [Abstract] Objective To determine the cerebral protective effect of glucose-6-phosphate dehydrogenase (G6PD) in rat early epilepsy. Methods Twenty-four SD rats were randomly and equally divided into 3 groups, normal control, model group and intervention group. The rats of intervention groups received an intraperitoneal injection of 0.1μg/ml 1, 25-dihydroxyvitamin D3 (1,25-(OH)2D3) for 7 d, and the animals of the normal control and model group were administered with an intracortical injection of normal saline. Rat models of epilepsy were established by intracortical microinjection of ferric chloride and identified by electroencephalography (EEG) in 2 h later and occurrence of seizures by behavior observation in 6 h later. Expression of G6PD mRNA and protein were detected in the cerebral cortex by RT-PCR and Western blotting. Malondialdehyde (MDA) level of lipid peroxidation and antioxidant level of glutathione (GSH) and G6PD activity were detected by colorimetric method. Results Typical seizures were observed in model and intervention groups. Th