硫链丝菌肽对人喉癌Hep-2 细胞生长及FoxM1 表达的影响.docVIP

硫链丝菌肽对人喉癌Hep-2细胞生长及FoxM1表达的影响 林力1，邓碧2，寿铸1，梁佳1，陈鸿雁1 （1.重庆医科大学附属第一医院耳鼻咽喉科 400016；2.重庆市渝北区人民医院麻醉科 401120） [通讯作者] 陈鸿雁，电话(023Email:cchhyy787@ [摘要]：目的 研究硫链丝菌肽(thiostrepton，TST)对人喉癌Hep-2细胞株增殖能力及FoxM1表达的影响。方法 用MTT法分别测定TST作用于人喉癌Hep-2细胞48h的增殖抑制率；通过流式细胞仪（FCM）检测不同浓度TST分别作用于Hep-2细胞48h后对其细胞周期动力学的影响；采用免疫细胞化学法、RT-PCR和Western blot检测Hep-2细胞中FoxM1的mRNA及蛋白表达以及不同浓度TST作用于Hep-2细胞48h FoxM1蛋白表达的变化。结果 不同浓度（1μM、2μM、4μM、8μM、12μM、16μM）TST作用于Hep-2细胞48小时后的增殖抑制率分别为5.27%、9.43%、20.25%、41.61%、67.96%、96.95%；浓度分别为2μM、3μM的TST作用于Hep-2细胞48h后，均使Hep-2细胞停滞于G1/G0期(P0.05)，且呈剂量依赖性(P 0.05)；同时将TST作用于Hep-2细胞48后，经免疫细胞化学法、RT-PCR和Western blot均检测到FoxM1mRNA及蛋白在Hep-2细胞中表达显著降低 (P 0.05)。结论 TST对人喉癌Hep-2细胞株具有明显的增殖抑制作用，其可能机制是与降低肿瘤细胞中FoxM1蛋白表达有关。 [关键词]：TST；FoxM1；Hep-2；细胞增殖 [中图法分类号文献标识码A Effect of thiostrepton on proliferation and FoxM1 expression in Hep-2 cell line Lin Li1,DengBi2,Shou Zhu1,Liang Jia1,Chen Hongyan1, (1.Department of Otolaryngology,First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016；2.Department of anesthesiology，the people′s Hospital of YuBei Distrcit,Chongqing 400090,China) Abstract: Objective To explore the effect of thiostrepton on proliferation and FoxM1 expression in Hep-2 cell line.Methods MTT reduction assay was used to investigate the proliferation inhibition of TST on Hep-2 cell line. The change of cell cycle after using TST were analyzed by flow cytometry；Immunocytochemistry RT-PCR and Western blot was used to investigate the FoxM1 expression in Hep-2 cell line by thiostrepton. Results the proliferation inhibition rate of TST on the Hep-2 cell line after 48h in each concentration group（1μM、2μM、4μM、8μM、12μM、16μM）are ()；Hep-2 cell line were kept at stage G1/G0 dose dependently in 1μM and 2μM group (P 0.05)；FoxM1 protein were supressed successfully with TST in 48h in Hep-2 cell line (P 0.05) .Conclusion Thiostrepton inhibits the proliferationand FoxM1 expression in Hep-2 cell line in a dose-dependent manner. 硫链丝菌肽(thiostrepton，TST)是一种噻唑类