毕业论文-梁磊.docxVIP

本科毕业论文题 目 鞘氨醇杆菌产酶条件的研究 学 院 食品科学与工程学院 专 业 生物工程 毕业届别 2016届 姓 名 梁 磊 指导教师 张卫兵 职 称 副教授 甘肃农业大学教务处制二〇一六年五月目录摘要......................................................................1关键词....................................................................1Abstract..................................................................1Key words.................................................................1前言......................................................................11材料与方法...............................................................21.1试验材料...............................................................21.1.1供试菌株.............................................................21.1.2主要仪器.............................................................21.1.3主要试剂.............................................................21.1.4培养基................................................................31.2试验方法...............................................................31.2.1初始pH值对菌株产酶的影响............................................31.2.2装液量对菌株产酶的影响...............................................31.2.3培养时间对菌株产酶的影响.............................................31.2.4培养温度对菌株产酶的影响.............................................31.2.5酵母浸粉含量对菌株产酶的影响.........................................31.2.6蛋白胨含量对菌株产酶的影响...........................................41.2.7 NaCl含量对菌株产酶的影响.............................................41.2.8羧甲基纤维素钠含量对菌株产酶的影响...................................41.2.9培养基组分含量的正交试验.............................................41.2.10最优发酵产酶条件的验证试验..........................................52结果与分析...............................................................52.1葡萄糖标准曲线的绘制...................................................52.2试验结果与分析.........................................................52.2.1培养基初始pH对菌株产酶的影响.........................................52.2.2装液量对菌株产酶的影响................................................62.2.3培养时间对菌株产酶的影响..............................................62.2.4