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qnrB4 qnrB1,10,42,53 qnrB6 文献中qnr引物 Target Name Sequence (5’→3’) Location Product size QnrS QnrS-A[1] QnrS-B CAATCATACATATCGGCACC AGTTCTTGCTGCCAGGCTGC 5165-5184 5751-5770 (qnrS1) 605 QnrB QnrB-A[1] QnrB-B ATGACGCCATTACTGTATAA GATCGCAATGTGTGAAGTTT 5270-5289 5812-5831 (qnrB1,10,42,53) 562 QnrA F[2] R GCAAGAGGATTTCTCACGCCAGG CAGTCGCGGGAGAAGGTGCCG 9006-9028 9462-9482 (qnrA1,7) 477 QnrB F[2] R ATGGCTCTGGCACTCGTTGG TCCATGAGCAACGATGCCTGG 5306-5325 5902-5922 (qnrB1,10,42,53) 617 参考文献 [1]周庭银.临床微生物诊断与图谱[M].上海：上海科学技术出版社， 2007.155-158,210-212. [2] Prathiba Kurupati,Carol Chow, Gamini Kumarasinghe,et al. Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR[J]. JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2004, p. 1337–1340. [3] Gladys Penn Siri, Nomathamsanqa Patricia Sithebe and Collins Njie Ateba. Identification of Klebsiella species isolated from Modimola dam (Mafikeng) North West Province – South Africa[J]. African Journal of Microbiology Research Vol. 5(23), pp. 3958-3963, 23 October, 2011 [4] A. Neuberger,I. Oren,and H. Sprecher. Clinical Impact of a PCR Assay for Rapid Identification of Klebsiella pneumoniae in Blood Cultures[J]. JOURNAL OF CLINICAL MICROBIOLOGY, Jan. 2008, p. 377–379. [5] Jane F. Turton, Claire Perry, Suzanne Elgohari,et al. PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets[J]. Journal of Medical Microbiology (2010), 59, 541–547. [6] Anna Ryberg, Crister Olsson, Siv Ahrné and Hans-Jürg Monstein, Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates, 2011, Journal of Microbiological Methods, (84), 2, 183-188. [7] Yin Liu a,b, Chao Liu a, Wenjie Zheng c,et al. PCR detection of Klebsiella pneumoniae in infant formula based on 16S–23S internal transcribed spacer[J]. International Journal of Food Microbiology 125 (2008) 230–235. [8] Cindy Fevre, Virginie Passet
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