第六章质谱分解.ppt

Molecular fragmentation by ion collisions with nitrogen molecules in ion optics Provides structural information for qualitative analysis Provides confirmatory ions for quantitative analysis Controlled via a single ion optics parameter -- fragmentor What is CID (Collision Induced Dissociation)? Capillary Fragmentation zone (CID) Skimmers Octopole Lenses Quadrupole M n [M] + 1 [M] 2 + DETAIL MS/CID/MS and LC/CID/MS LC/CID/MS Stage 2 API ion source Stage 3 Fragmentation Mass analysis Stage 1 HPLC LC separation [M ] 4 + [M ] 1 + [M ] 2 + M n MS/CID/MS Stage 2 Stage 3 Stage 1 Mass separa