《大野荞清除活性氧作用研究本科毕业论文》.docVIP

大野荞清除活性氧作用研究 摘 要：用NBT的光还原原理、Fenton体系产生的羟自由基与番红花红T作用后使体系吸光度降低、H2O2能和钼酸铵反应形成黄色络合物等原理研究了大野荞各器官（花、叶、茎、根、种子）水提液对羟基自由基（·OH）、超氧阴离子自由基（·O2–）和过氧化氢(H2O2)的清除作用。结果发现：花对·OH的清除作用极显著高于种子，与叶、茎之间无显著差异；根对·O2-的清除作用与花无显著差异，但都极显著高于叶、种子和茎；叶对H2O2的清除作用与花无显著差异，但都极显著高于根、种子和茎。 关键词：大野荞；·OH；·O2-；H2O2；活性氧 Scavenging of Reactive Oxygen Species by the Extraction of F. megaspartanium Abstract: This experiment used the method of nitro blue terra-zolium photochemical reduction, the system of fenton can produce the·OH, which can function with Saffron red T ,and then this system reduces the absorbance, H2O2 and ammonium molybdate can become the yellow soil thing and so on, to study the scavenging of the hydroxyl radical (·OH) , superoxide anion radicals (·O2 - ) and hydrogen peroxide ( H2O2 ) were estimated by aqueous extractions from various organs (flower, leaf, stem, root, seed)of F. megaspartanium. The results showed that: the cleaning effect of the flower on ·OH was more significant than the seed, the flower was not significant in difference between the leaf and stem; The root was not significant in difference in the scavenging effect of ·O2 - compared with the flower, but which was more significant than the leaf, seed and stem; The leaf was not significant in difference in the scavenging effect of H2O2 compared with the flower, but which was more significant than the root, seed and stem. Keywords: F. megaspartanium; ·OH; ·O2-; H2O2; Reactive oxygen species 目 录 引言 1 1. 材料和方法 2 1.1 供试材料 2 1.2仪器与试剂 2 1.3方法 2 2. 数据处理 5 3. 结果与分析 5 3.1大野荞各器官水提液对·OH的清除作用 5 3.2大野荞各器官水提液对·O2-的清除作用 6 3.3大野荞各器官水提液对H2O2的清除作用 6 4. 讨论 7 参考文献 9 致 谢 11 引言 荞麦属于蓼科(Polygonaceae)荞麦属(Fagopyrum)，全世界已见报道并正式命名的有23个种、3个变种、2个亚种和1个人工合成种，分为大粒和小粒两个组，以大粒组的荞麦较为重要[1]。荞麦在世界上分布较广，中国、俄罗斯、日本、韩国、尼泊尔、不丹、印度、波兰、匈牙利、斯洛文尼亚等是其主产国和主要利用国。我国是世界荞麦主要产地之一，我国的西南地区，分布有大量的野生荞麦种类[2,3]。荞麦富含蛋白质、淀粉、粗纤维、维生素、矿物元素等，并含有一些其他粮食作物不含或少含的营养物质，蛋白质的氨基酸组成十分平衡，与人类的营养非常吻合，能有效预防糖尿病、心血管硬化、高血压，具有健胃消食、增强免疫力，促进小孩生长及智力发育等功效。此外，荞麦还具有预防癌症的功效