=中国林蛙抗菌肽的提取及其抗菌活性的研究.docVIP

中国林蛙皮抗菌肽的提取及其抗菌活性的研究 王战勇1，1，苏婷婷2 (1. 辽宁石油化工大学环境与生物工程学院，辽宁抚顺 113001；2. 辽宁石油化工大学化学与材料学院，辽宁抚顺 113001) 摘要: 采用醇提。考察了影响的各个因素如提取时间提温度料液比等因素，通过正交试验确定的最佳条件。即：提取时间为h，提取温度为℃，提取料液比为1。此条件下可达%。对。对各测试菌株的最低抑制浓度分别为：金黄色葡萄球菌和链球菌为0.25mg/mL；枯草芽孢杆菌和大肠杆菌为0.5mg/mL；蜡样芽胞杆菌为1.0mg/mL；白色念珠菌、酿酒酵母和毛霉为2.0mg/mL；根霉为4.0mg/mL。 关键词：中国林蛙；抗菌肽；；抑菌活性 中图分类号：Q516文献标识码: A The Study on Extraction and Antimicrobial Activity of Antimicrobial Peptides from Skin of Rana chensinensis WANG Zhanyong1, 1, SU Tingting2 (1. School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, China；2. School of Chimical and Material Sciences, Liaoning Shihua University, Fushun 113001, China) Abstract: Antimicrobial eptides were isolated from Skin of Rana chensinensis by methanol. Some factor of influence on antimicrobial percentage of antimicrobial peptides crude product was reviewed, such as time, temperature, ratio of sample and extraction solvent and pH. The optimum condition was chosen by orthogonal experiment. The optimum conditions are as follows: The ratio of sample and extraction solvent is 1: 25. The extracting solution was disposed for 24h at -20℃. And the pH of extracting solution is 5.0. Under the optimize conditions antimicrobial percentage of antimicrobial peptides can reach 75.22%. Antimicrobial peptides crude product could effectively inhibit the growth of series microorganisms, including Gram-positive bacteria, Gram-negative bacteria and fungus. The MICs of the tested microorganisms were tested as follows: The MIC of Staphylococcus aureus and Streptococcus is 0.25mg/mL, the MIC of Bacillus subtilis and Escherichia coli is 0.5mg/mL, the MIC of Bacillus cereus is 1.0mg/mL, the MIC of Candida albicans, Saccharomyces cerevisia and Mucor is 2.0mg/mL, the MIC of Rhizopu is 4.0mg/mL. Key words: Rana chensinensis, Antimicrobial Peptides Orthogonal experiment, Antimicrobial Activity 两栖动物皮肤在自然进化过程中形成了防御病原微生物的三套防御系统，相应地具有与这些防御系统相适应的特定结构。皮肤抗菌肽是其中先天防御系统的主要组成部分。两栖动物皮肤抗菌肽具有高效、广谱、不易产生耐药性等特点[