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白藜芦醇对高脂血症大鼠血小板聚集的影响及其机制研究 白云霞 杭州市第一人民医院，浙江杭州 310004 中图分类号：R363.2文献标识码：A 摘要： 研究白藜芦醇 建立大鼠的高脂血症模型，同时连续ig 给予白藜芦醇·d）或空白溶媒30d，测定大鼠血浆总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、丙二醛、超氧化物歧化酶，一氧化氮、内皮一氧化氮合酶P-选择蛋白，血栓烷B2、和6-酮-前列腺素F1α，ADP诱导的血小板5分钟最大聚集率 与饲高脂饲料组比较，饲高脂血症大鼠在连续ig给予白藜芦醇·d）30 d后，大鼠血浆TG、TC、LDL-C含量平均下降，分别下降19%,31%,51%，1.33倍一氧化氮合酶一氧化氮和6-酮-前列腺素F1α含量增加，P-选择蛋白、血栓烷B2含量降低，ADP诱导的血小板5分钟最大聚集率白藜芦醇血浆或组织中的TXA2和PGI2平衡及细胞内外的钙离子平衡白藜芦醇 Study of the effects and the mechanismof resveratrol on platelet aggregation in hyperlipidemia rats Bai Yun-Xia, Hang zhou first hospital, Hangzhou 310000 Abstract: Aim To explore the effects and the mechanism of resveratrol on platelet aggregation in hyperlipidemia rats. Methods Experimental hyperlipidemia rats were ig administration of resveratrol 50mg/kg·d or vehicle in 30 d. The levels of plasma triplyceride TG, total cholesterol TC, high density lipoprotein cholesterol HDL-C, low density lipoprotein cholesterol LDL-C, malondialdehyde MDA, superoxide dismutase SOD，nitric oxide NO, endothelial nitric oxide synthase eNOS, granulemembrane protein-140（GMP-140），thromboxaneB2 TXB2，6-keto –prostaglandin F1α 6-keto-PGF1α and platelet maximal aggregation rate（MAR）which was induced by ADP in 5 minute were measured. Results Plasma TG, TC,LDL-C and HDL-C of the rats fed high fat diet HFD and ig resveratrol 50mg/kg·d was 19%， 31%，51% lower and1.33times higher than that of the HFD group rats. The activity of SOD and eNOS in resveratrol and HFD group was higher P0.01 and The content of NO and 6-keto-PGF1α in resveratrol and HFD group was higher P0.01 and the MDA、 GMP-140、TXB2 and MAR in resveratrol and HFD group was lower than that of the HFD group P0.05. Conclusion Platelet aggregation rate was descreased in experimental hyperlipidemia rats by i.g. administration of resveratrol. its mechanism closely correlates with decline of plasma lipid and anti- peroxide of lipid and protecting vasoendothelial cells and improving the synthesis of NO as well as Keeping t