三氯乙烯致超敏性皮炎与肝代谢酶基因和免疫相关基因的关系研究(SCI论文)_图文.docVIP

三氯乙烯致超敏性皮炎与肝代谢酶基因和免疫相关基因的关系研究(SCI论文)_图文 Toxicology Research Citethis:Toxicol.Res.,2016,5,633 Trichloroethylene-inducedhypersensitivity dermatitiswasassociatedwithhepaticmetabolicenzymegenesandimmune-relatedgenes XinyunXu,*YuebinKe,JianhuiYuan,YuefengLiu,XueyuLi,DeshengWu,XiaoyunQin,JiyanMaoandKanlangMao Trichloroethylene(TCE)isoneofthecommonorganicsolventsthathasbeenwidelyusedincleaningordegreasingofmetalandelectronicproducts.However,hundredsofcasesofhypersensitivitydermatitishaveoccurredaftertheworkerswereoccupationallyexposedtoTCEinChinaoverthepastdecade.ThepurposeofthisstudywastoinvestigatemRNAexpressionofhepaticmetabolicenzymegenes,immune-relatedgenes,apoptosisgenesandoncogenesinpatientswithhypersensitivitydermatitisinducedbytrichloroethylene.12typicalpatientswithTCE-inducedhypersensitivitydermatitiswereinvestigatedasthestudycases,peripheralbloodsamplesweretakenfrompatientsandcontrol,andreal-time?uor-escencePCRassaywasappliedfordetectionofmRNAexpressionofhepaticmetabolicenzymegenes,immune-relatedgenes,apoptosisgenesandoncogenes.ItwasfoundthattherelativelevelsofmRNAexpressionofCYP1A2,CYP2E1,CYP3A4andCYP2C9increasedby723%,318%,385%and216%,respect-ively,whencomparedwithcontrol(plt;0.01orplt;0.05);Foxp3,GATA3andCTLA4mRNAexpressionincreasedby104%,106%and253%,respectively,inTCEpatientswhencomparedwithcontrol(plt;0.01);T-betexpressio