兔颈总动脉破裂粥样硬化斑块模型炎症因子的表达.docVIP

兔颈总动脉破裂粥样硬化斑块模型炎症因子的表达 　　 　　 　　[摘要] 目的 探索兔颈总动脉破裂粥样硬化斑块模型血清炎症因子的水平。 方法 将30只健康雄性新西兰白兔随机分为3组，对照组（n=10）、高脂饮食组（n=10）和球囊损伤组（n=10）。饲养8周后，采用“腔外负压”法诱导斑块破裂，观察兔血清脂蛋白、白介素-6及hs-CRP水平的变化，彩色多普勒超声鉴定斑块形成。 结果 高脂饲养8周后，球囊损伤组、高脂饮食组的血清脂蛋白水平、颈总动脉血流速度、IMT显著高于对照组（P＜0.05），而颈总动脉内径低于对照组；血清IL-6、hs-CRP水平显著高于对照组，高脂饮食组与球囊损伤组间差异有统计学意义。 结论 炎症反应参与动脉粥样硬化的形成，在粥样硬化斑块破裂中扮演重要角色；为破裂斑块的形成提供了一定的动物模型理论基础。 　　[关键词] 兔；动物模型；颈动脉粥样硬化斑块；炎症因子 　　[中图分类号] R743[文献标识码] A[文章编号] 1674-4721（2014）05（b）-0004-04 　　 　　The express of inflammatory factor in animal model of carotid atherosclerosis plaque rupture 　　LIU Xin-ye YANG Dong-wei▲ CHENG Yong LIU Min CHI Hao 　　Department of Cardiology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450007,China 　　[Abstract] Objective To explore the express of inflammatory factor in animal model of carotid atherosclerosis plaque rupture. Methods 30 healthy New Zealand white male rabbits were randomly divided into 3 groups including control group (n=10),carotid lesion group with balloon dilation (n=10) and the high fat-diet group (n=10).The concentrations of serum lipoprotein,interleukin-6,hypersensitive C reactive protein,internal diameter of carotids,intima-media thickness and blood flow rate of carotids were obtained used by color Doppler ultrasound respectively,plaque ruptures were triggered by negative pressures of intra-cavity in carotids injury group,the relationship among inflammatory factor,the severity of atherosclerosis and the achievement ratio of carotid atherosclerosis plaque rupture were evaluated. Results 8 weeks after the high-fat feeding,the concentrations of plasma lipoprotein,inflammatory factor,intima-media of carotids and blood flow rate of carotids were significant increase in high fatty combining with carotids injury group and diet group received alone compared with control group (P0.05).Intima-media of carotids was not smooth and internal diameter was decreased than control group,there had significant difference between high fatty