人源抗HER2单链抗体-轻链恒定区-鱼精蛋白截短体融合蛋白的基因构建、表达及活性鉴定.docVIP

人源抗HER2单链抗体/轻链恒定区/鱼精蛋白截短体融合蛋白的基因构建、表达及活性鉴定 作者：王凯，温伟红，王涛，张瑞，秦炜炜，雷小英，杨安钢 【关键词】 HER2；,单链抗体；,鱼精蛋白 Construction and expression of human antiHER2 ScFv/Ck/tP fusion protein in and identification of its activity 【Abstract】 AIM: To construct a fusion protein gene of human antiHER2 ScFv/light chain constant region/truncated protamine and analyze the binding activity of the expressed protein. METHODS: Two pairs of oligonucleotide primers were designed and used to amplify the ScFv and the Ck gene. After they were linked as ScFv/Ck, the