HMGB1基因多态性与HBV相关性肝癌的关联性研究.docVIP

HMGB1基因多态性与HBV相关性肝癌的关联性研究 　　【摘要】 目的：探讨高迁移率族蛋白B1（HMGB1）第4内含子1176G/C与HBV感染后肝癌是否存在关联。方法：采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法检测110例HBV感染后肝癌患者及316例HBV感染后非肝癌患者HMGB1 1176G/C多态性，采用 字2检验及非条件logistic回归统计方法进行分析。结果：乙肝肝癌组3种基因型及G、C等位基因分布与慢性乙型肝炎组比较差异有统计学意义（字2=6.152，P=0.046；字2=5.605，P=0.018）。在显性模式下乙肝肝癌组与慢性乙型肝炎组比较差异有统计学意义（P=0.023），在隐性模式下与乙肝病毒携带组、轻型肝病组比较差异有统计学意义（P=0.048，P=0.028），在共显性模式下与轻型肝病组比较差异有统计学意义（P=0.03）。结论：HMGB1基因多态性与HBV感染后肝癌易感性相关。 　　【关键词】 高迁移率族蛋白B1； 单核苷酸； 乙型肝炎病毒； 多态性； 肝癌细胞 　　The Correlation of Genetic Polymorphisms of HMGB1 to Hepatitis B Virus-related Hepatocellular Carcinoma/WANG Dan，DENG Chun-qing，ZHAO Long-feng.//Medical Innovation of China，2014，11（12）：013-016 　　【Abstract】 Objective： To study the possible association of the genetic polymorphism of high mobility group protein B1 （HMGB1） 1176 G/C intron 4 with the susceptibility to hepatocellular carcinoma （HCC）after hepatitis B virus （HBV） infection. Method： 110 patients with HBV-related HCC and 316 patients of HBV infection without HCC after HMGB1 intron4 1176G/C polymorphism were detected by polymerase chain reaction-restriction fragment length pdymo-rphism（PCR-RFLP）method， chi-square test and unconditioned logistic regression model were applied to analysis results. Result： There were significant statistically difference in the three genotypes among the HBV-related HCC group and the chronic HBV infection group（字2=6.152，P=0.046；字2=5.605，P=0.018）. There were significant difference （P=0.023） between HCC and CHB under the dominant model either， hepatitis group of AsC，AsC+CHB different from HCC group（P =0.048， 0.028） under the recessive model respectively. There were significant difference between HCC and AsC+CHB under codominant model（P=0.03）. Conclusion： The results suggested that the genotype of HMGB1 intron4 1176 G/C is associated with the susceptibility to HBV-induced hepatocellular carcinoma. 　　【Key words】 High mobility group protein B1； Mononucleotide； Hepatitis B virus； Polymorphisms； Hepatocellular carcinoma