转染微小RNA―132对骨肉瘤SaOS―2细胞生长和凋亡的作用.docVIP

转染微小RNA―132对骨肉瘤SaOS―2细胞生长和凋亡的作用 　　摘要：目的 观察转染微小RNA-132 （miR-132）对体外人骨肉瘤细胞株SaOS-2生长和凋亡的影响，并初步探讨其作用机制。方法 实时荧光定量逆转录-聚合酶链反应（RT-qPCR）检测34例骨肉瘤组织及癌旁正常组织中miR-132 mRNA的表达；Cell Counting Kit-8 （CCK-8）法检测细胞增殖；细胞经DAPI染色后在荧光显微镜下观察细胞凋亡；Western blot检测体外SaOS-2细胞中Mucin-4、HER-2和p-FAK蛋白的表达。结果 miR-132 mRNA在骨肉瘤组织中的表达明显低于癌旁正常组织，差异有统计学意义（P 0.05）；与对照组相比较，转染组细胞中miR-132 mRNA的表达明显升高，细胞??殖被明显抑制，凋亡细胞显著增加，差异均有统计学意义（P 0.05）；Mucin-4、HER-2和p-FAK蛋白在转染组细胞中的表达均下调，与对照组相比较，均有统计学差异（P0.05）。结论 转染miR-132对体外骨肉瘤细胞有增殖抑制和凋亡诱导作用，miR-132有望成为骨肉瘤治疗的新靶点。 　　关键词：骨肉瘤；miR-132；凋亡 　　Effects of MicroRNA-132 Transfection on the Proliferation and Apoptosis in Osteosarcoma SaOS-2 Cell Line in Vitro 　　ZHOU Qi 　　（The First People Hospital of Yueyang，Yueyang 414000， Hunan，China） 　　Abstract：Objective To observe the biological role and underlying mechanism of miR-132 in osteosarcoma proliferation and apoptosis. Methods The expression of miR-132 mRNA in the cancer tissue and their adjacent tissues in 34 osteosarcoma patients were detected by real-time quantitative polymerase chain reaction （RT-qPCR）. The biological effects of miR-132 transfection on osteosarcoma SaOS-2 cells were assessed by CCK-8 assay and DAPI staining. Western blotting was used to detect the expression of Mucin-4， HER-2 and p-FAK in SaOS-2 cells. Results The expression level of miR-132 mRNA was found to be downregulated in osteosarcoma tissues compared with the matched adjacent tissues. After transfection， the expression of miR-132 mRNA was significantly higher than control group. The proliferation of SaOS-2 cells was inhibited significantly by miR-132 transfection. Apoptosis rate induced by the miR-132 transfection was markedly higher than that of control. The proteins of Mucin-4， HER-2 and p-FAK were decreased after miR-132 transfection. Conclusion miR-132 is downregulated in osteosarcoma tissues， transfected of miR-132 can effectively inhibit proliferation and promote apoptosis of SaOS-2 cells in vitro， miR-132 may become a new target for the regulat