USP30NF25_197分光光度鉴别检验.docVIP

197 SPECTROPHOTOMETRIC IDENTIFICATION TESTS 197分光光度鉴别检验 Spectrophotometric tests contribute meaningfully toward the identification of many compendial chemical substances. The test procedures that follow are applicable to substances that absorb IR and/or UV radiation (see Spectrophotometry and Light-Scattering 851). 分光光度法检测对于很多药典化学物质的鉴别做出了意味深远的贡献。下面的这些步骤适用于那些吸收红外和/或者紫外线的物质（见分光光度测定法和光散射851）。 The IR absorption spectrum of a substance, compared with that obtained concomitantly for the corresponding USP Reference Standard, provides perhaps the most conclusive evidence of the identity of the substance that can be realized from any single test. The UV absorption spectrum, on the other hand, does not exhibit a high degree of specificity. Conformance with both IR absorption and UV absorption test specifications, as called for in a large proportion of compendial monographs, leaves little doubt, if any, regarding the identity of the specimen under examination. 一个物质的红外吸收光谱，在与从对应的USP标准品处获得的光谱图进行比较后，或许提供了从任何单一检验中所能获得的关于该物质的鉴别的最具决定性的证据。而另一方面，紫外吸收图谱则并未展示出高度的特异性。与红外吸收和紫外吸收检验标准的符合性，如在很大比例的药典专论中所要求的，用于供试样品的鉴别几乎不会导致任何质疑。 INFRARED ABSORPTION 红外吸收 Six methods are indicated for the preparation of previously dried test specimens and Reference Standards for analysis. The reference 197K in a monograph signifies that the substance under examination is mixed intimately with potassium bromide. The reference 197M in a monograph signifies that the substance under examination is finely ground and dispersed in mineral oil. The reference 197F in a monograph signifies that the substance under examination is suspended neat between suitable (for example, sodium chloride or potassium bromide) plates. The reference 197S signifies that a solution of designated concentration is prepared in the solvent specified in the individual monograph, and the solution is examined in 0.1-mm cells unless a different cell path length is specified in the individual monograph. The reference 197A signifies that the subs