生基因表达的调控概述.pptVIP

(A) Plac-lacZ expression was characterized for wild-type NCM3722 cells growing exponentially on glycerol, glucose, or lactose as the sole carbon source (red diamonds, squares and triangles, respectively); 1 mM IPTG was added to deactivate LacI. At time zero, 20 mM oaa was added; a transient repression period of ~30 min is shaded in grey. Strain NQ1053 carrying PlacUV5-lacZ (black squares; right y-axis) was not affected. (B) LacZ expression levels before and during the repression period are quantified and shown as the red and black bars. Also quantified are the expression levels of two PTS de