CORN GEM PROJECT Iowa State University爱荷华州立大学玉米与创业板项目.pptVIP

Background Corn breeding program: crossing and selecting for oil and protein. Stiff Stalk crossed with germplasm from Argentina. Non- Stiff Stalk crossed with germplasm from St. Croix. Developed lines of different oil and protein content. Objectives To characterize the inbred lines for seed quality. To evaluate the effect of oil and protein levels on seed quality. To evaluate the effect of genetic background and production location on seed quality. Material and Methods Seed production Ames-IA Clinton-IL Colombia-MO Hooper-NE Starkville-MS Standard Germination Test Leakage of electrolytes: amino acids and organic acids. Poor membrane integrity in deteriorated, low vigor seeds. EC Imbibe seeds in deionized water for 24 hs at 25o C Individual Seed Analyzer Increased temperature Temperature: 42o C for 96h High relative humidity Planting: Over CCP covered with sand Other Tests Soak test Fast green (FG) 100 seed weight Inbred quality index (IQI) SC+AA+FG+Soak + [ 200- (EC6h+EC24h)] 60 Table 2. Levels of significance from ANOVA for ten maize inbred lines evaluated in four locations. Table 3. Seed vigor tests comparisons between groups of lines bulked by exotic genetic background, Argentina (AR) and St. Croix (SC), and seed composition, high oil (HO), low oil (LO). Conclusions Low protein lines have the lowest seed vigor. When protein levels were high, seed quality was high regardless of oil content. Mo17 and B73 have the highest IQI. Inbred Quality Index results suggests that seed quality attributes should be considered in a comprehensive breeding program. Inbred line 4 (high protein) has the highest vigor when produced in IA and NE. Conclusions Inbred lines with Argentina germplasm have the highest seed quality. Seeds produced in IL have the best quality, while those produced in MO have the lowest quality. Acknowledgements Tom Hoegemeyer- NE Kevin Montgomery- IL David Willmot - MO Paul Williams- MS Penny Meyerholz Phillip Dixon (ISU Stats