IsaPotentialMediatorandMarkeroftheTumorInvasionof.ppt

Secreted CXCL1 Is a Potential Mediator and Marker of the Tumor Invasion of Bladder Cancer by Hiroaki Kawanishi, Yoshiyuki Matsui, Masaaki Ito, Jun Watanabe, Takeshi Takahashi, Koji Nishizawa, Hiroyuki Nishiyama, Toshiyuki Kamoto, Yoshiki Mikami, Yoshinori Tanaka, Giman Jung, Hideo Akiyama, Hitoshi Nobumasa, Parry Guilford, Anthony Reeve, Yasushi Okuno, Gozoh Tsujimoto, Eijiro Nakamura, and Osamu Ogawa Clin Cancer Research Volume 14(9):2579-2587 May 1, 2008 ?2008 by American Association for Cancer Research A, invasion of bladder cancer cell lines in a modified Boyden chamber. Hiroaki Kawanishi et al. Clin Cancer Res 2008;14:2579-2587 ?2008 by American Association for Cancer Research A, cDNA microarray data for CXCL1. Hiroaki Kawanishi et al. Clin Cancer Res 2008;14:2579-2587 ?2008 by American Association for Cancer Research A, knockdown of CXCL1 by RNAi vector in T24 cells was confirmed by ELISA. Values were corrected for the total protein in the cell lysate. Hiroaki Kawanishi et al. Clin Cancer Res 2008;14:2579-2587 ?2008 by American Association for Cancer Research A, expression of MMP-13 mRNA in T24 and RT112 cells in which CXCL1 was engineered to be repressed or overexpressed. Hiroaki Kawanishi et al. Clin Cancer Res 2008;14:2579-2587 ?2008 by American Association for Cancer Research A, CXCL1 levels in the urine of controls and patients with bladder cancer. Hiroaki Kawanishi et al. Clin Cancer Res 2008;14:2579-2587 ?2008 by American Association for Cancer Research A, invasion of bladder cancer cell lines in a modified Boyden chamber. Cells (5 × 104) were seeded into the upper compartment of the chamber in serum-free RPMI 1640 and incubated for 24 h at 37°C. RPMI 1640 with 10% FCS filled the lower compartment. Columns, mean of the number of cells in the five densest spots identified on the lower surface of the filter within a single ×200 field in each of three experiments; bars, SD. B, reverse-phase chromatograms of cell culture supernatants. Eluted proteins w