JES-sampleJES-sample.doc

Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana Shaohua (given name) Wang (family name)1,2, Ping Wang2,* Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. E-mail: jesc@263.net (first author’s email address) 2. School of Life Sciences and Technology, Jinan University, Guangzhou 510632, China Received 06 November 2006; revised 12 December 2006; accepted 24 January 2007 Abstract: DNA damage in the form of cyclobutane pyrimidine dimers (CPDs) (Abbreviations should be explained when it is shown first time in following three parts: Abstract, main text part, and figure and table. BTW, if it only shown once in the Abstract, it is not necessary using abbreviation in the Abstract) and photoproducts (6--4 PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using the technologies with specific monoclonal antibodies. CPDs and 6--4 PPs increased during 3 hr UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6--4 PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0 and 12℃, with 50% CPDs removal during 1 hr exposure to white light. Photorepair of 6--4 PPs at 12℃ was very slow as compared with that at 24℃, and almost no removal of 6--4 PPs was detected after 4 hr exposure to white light at 0℃. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications. Key words: cyclobutane pyrimidine dimers; Arabidopsis thaliana; DNA repair ---------------------------------- * Corresponding author. E-mail: jesc@263.net Introduction There is growing awareness of the potential biological effects of stratospheric ozone depletion