生物信息学实验三、核酸序列、常用分子信息学软件使用要点.doc

实验三、核酸序列分析、常用分子信息学软件使用 1 、用BioEdit等软件进行序列分析 用BioEdit打开FASTA格式的序列 Sequence--Nucleic acid-- 1）Sequence--Nucleic acid--Nucleotid composition DNA molecule: gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA Length = 744 base pairs Molecular Weight = 227718.00 Daltons, single stranded Molecular Weight = 453776.00 Daltons, double stranded G+C content = 61.56% A+T content = 38.44% Nucleotide Number Mol% A 141 18.95 C 251 33.74 G 207 27.82 T 145 19.49 2）Sequence--Nucleic acid--Base composition and mass export(monoisotopic) Title LENGTH A G C T Gap Other Mono_exact_mass BaseComp OppositeStrand_exact_mass OppositeStrand_BaseComp gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA 744 141 207 251 145 0 0 228830.3528025 A141 G207 C251 T145 230626.6695859 A145 G251 C207 T141 3)Sequence--Nucleic acid--Base compoeition and mass export with average masses Title LENGTH A_COUNT G_COUNT C_COUNT T_COUNT GAP (not used in db) OTHER_COUNT MONO_EXACT_MASS BASE_COMP OPPOSITE_STRAND_MASS OPPOSITE_STRAND_BASE_COMP SEQUENCE (not used in db) OppositeStrand_Ave_mass OppositeStrand_BaseComp gi|725605238|ref|XM_010330964.1| PREDICTED: Saimiri boliviensis boliviensis interferon, lambda 3 (IFNL3), mRNA 744 141 207 251 145 0 0 228939.3442020 A141 G207 C251 T145 230736.4659940 A145 G251 C207 T141 4）Sequence--Nucleic acid--complement CACTGTCGGAGTCTCACCCCAGGAAGACGACTGCTTCTGGTCTCTAGTCTTTACTTTGCTCCGTACTCGTCCCCGACGTACCGTCACGACCACGACTACCGGTGTCACGACTGACAGTGACCTCGTCAAGGACAGTGGTCCGGGGGGTCCCGGGAGGGCCTACGTTCCCCGACGGTGTATCGGGTCAAGTTCAGAGACAGGGGTGTCCTCGACGTTCGGAAATTCTCTCGGTTTCTACGGAATCTTCTCAGCGAAGACGACTTCCTGACGTCCACGGCGAGGGCGGAGAAGGGGTCCTGGACCCTGGACTCCGTCGACGTCCACTCCCTCGCGGGGCACCGGAACCTCCGACTCGACCGGGACTGCGACCTCCAAGACCTCCGGTGGCGACTGTTACTGTACCGGGACCCACTGCAGGACCTGGCCGGGGAAGTGTGGGACGTGGTGCAGGAGAGGGTCGAGGCCCGGACACAGGTC