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系统性红斑狼疮患者血清DNA酶测定的临床意义系统性红斑狼疮患者血清DNA酶测定的临床意义中华皮肤科杂志 1998年第6期第31卷 论著作者：曾凡钦　陈宜芳　李伯有　林宝珠　许德清单位：510120 广州，中山医科大学孙逸仙纪念医院皮肤科关键词：红斑狼疮；系统性；脱氧核糖核酸酶类【摘要】　目的　研究血清DNA酶(DNase)浓度异常在系统性红斑狼疮(SLE)发生发展中所起的作用。方法　于国内首次用单相酶扩散法检测了63例SLE患者。结果　患者血清中DNase浓度明显低于正常人；活动期患者明显低于缓解期患者；SLE伴肾损害患者低于非肾损害患者。血清DNase的浓度下降与DNA含量升高呈负相关；与血沉、ANA呈负相关；与补体C3、C4呈正相关；与病情活动积分呈负相关。结论　SLE患者由于其DNase水平低下，使得血循环中含DNA的自身抗原长期滞留，发挥免疫原性，并与抗DNA抗体结合形成免疫复合物，造成免疫损伤。患者血清DNase水平低下在SLE的发病机制中起着一定作用。The Measurement of Deoxyribonuclease and Its Clinical Significance in Patients with SLEZeng Fanqin, Chen Yifang, Li Boyou, et al. Department of Dermatology, Sun Yat?sen Memorial Hospital, Sun Yat?sen University of Medical Sciences, Guangzhou 510120【Abstract】　Objective　To study the role of abnormal concentration of DNase in the development of SLE.Methods　The concentration of DNase was measured by single radial enzyme diffusion method in the sera of 63 SLE patients.Results　The concentration of DNase in the patients was significantly lower than that in healthy inpiduals, significantly lower in patients at active stage than that in remission, and lowest in patients with lupus nephritis. The decrease of serum DNase was negatively correlated with the increase of serum DNA. The level of DNase was negatively correlated with ANA and ESR, positively correlated with C3, C4, and negatively correlated with the severity of the disease.Conclusion　The above data suggest that the decrease of serum DNase makes the persistence of DNA in the blood circulation which combines with antiDNA antibody to form immune complex, resulting in autoimmune lesions. The decrease of serum DNase may play an important role in the pathogenesis f SLE.【Key words】　Lupus erythematosus, systemic　Deoxyribonucleases已经证实系统性红斑狼疮(SLE)患者血循环中DNA水平升高，但其升高的原因尚不清楚，有可能是因为DNA清除机制的缺陷。我们用单相酶扩散法检测SLE患者血清DNA酶(DNase)的浓度，并探讨血清DNase浓度异常与DNA升高的关系以及在SLE发生发展中所起的作用。材料与方法1.主要试剂：三羟甲基氨基甲烷(Tris)、牛胸腺DNA、牛胰DNA酶Ⅰ(DNase)、RNA酶A(RNase)：Fluka公司和Sigma公司产品；溴化乙啶(Ethidium bromide, EB)、琼脂糖：华美生物工程公司产品。2.仪器：荧光分光光度计：F-20