人胎盘组织源造血干-祖细胞的初步研究.docVIP

人胎盘组织源造血干/祖细胞的初步研究 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：刘玉峰，张永卓，张传新，王叼 【摘要】nbsp; 为了探讨足月胎盘组织源单个核细胞(human mature placenta tissue-derived mononuclear cells，hPTMNC)中CD34+细胞的体外增殖、分化能力，寻找新的造血干/祖细胞来源供临床应用，分别采用流式细胞术检测、造血干/祖细胞集落培养和体外无细胞因子长期培养的方法，测定胎盘组织源和脐血中的CD34+细胞及其亚群和集落形成单位的数量。结果表明：胎盘组织源CD34+细胞（2.74±0.61%）及其亚群CD34+/ CD38-细胞（2.46±0.42%）、造血干/祖细胞集落CFU-GM（186.90±24.52）和BFU-E（101.40±13.35）水平都较脐血CD34+细胞（1.73±032%）、CD34+/CD38-细胞（0.80±0.25%）、CFU-GM（136.90±25.15）、BFU-E(49.20±8.13)高；前者在体外无细胞因子培养条件下存活时间长，且细胞数量增加约2倍。结论：胎盘是胎儿期的造血器官，胎盘细胞成分更幼稚，是一种造血干/祖细胞移植的新的种子细胞。 【关键词】nbsp; 胎盘组织 　　Preliminary Study on Human Mature Placenta Tissue-derived Hematopoietic Stem/Progenitor Cells 　　Abstractnbsp;nbsp;nbsp; Clinical transplantation has indicated that cord blood (CB) can be used in the hematopoietic reconstitution in the children，but not well used in the adult patients because of the low cell amount. The present study aimed to explore the capability of proliferation and differentiation of the hematopoietic stem /progenitor cells derived from human mature placenta tissuenbsp; (PT) in vitro，andnbsp; to find a new source of hematopoietic/progenitor cells for clinical transplantation.nbsp; CD34+ cellsnbsp;nbsp; in human mature placenta tissue were isolated and characterizednbsp; by using enzyme-digestion method and flow cytometry. A long culture system without cytokines was established with human mature placenta tissue-derived mononucleated cells and cord blood mononuclear cells. The number of nucleated cells was weekly counted in culture for 14 weeks. The number of CFC was counted in culture for 2 weeks.nbsp; The results showed thatnbsp; the CFC yields（CFU-GM，186.90±24.52；BFU-E，101.40±13.35）and the percentage of CD34+cells (2.74±0.61%) and CD34+/CD38- cells (2.46±0.42%)nbsp; in placenta tissue (PT) were higher than CFC（CFU-GM，136.90±25.15；BFU-E，49.20±8.13），CD34+ cells (1.73±0.32%)nbsp; andnbsp; CD34+/CD38- cells (0.80±0.25%) in cord blood (CB). T