六味地黄丸对oletf大鼠胰腺凋亡相关基因bcl.docVIP

六味地黄丸对OLETF大鼠胰腺凋亡相关基因bcl nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：薛耀明，罗仁，朱波，张燕，潘永华，李晨钟 【关键词】nbsp; 糖尿病 nbsp;nbsp;nbsp; ［摘要］nbsp; 目的：探讨六味地黄丸对自发性2型糖尿病大鼠胰腺组织凋亡相关基因bcl2和Bax表达的影响。方法：OLETF大鼠（自发性2型糖尿病）40只，随机分为六味地黄丸治疗组和模型组，每组20只；另同系LETO大鼠（非糖尿病）10只作为正常对照组。六味地黄丸治疗组于大鼠8周龄起，用六味地黄丸按2.4 g#12539;kg1#12539;d1灌胃，余组用等量蒸馏水灌胃。每周记录大鼠体质量；采用口服葡萄糖耐量试验监测血糖；定期处死大鼠，分离胰腺并称重；采用逆转录聚合酶链反应检测bcl2和Bax在胰腺组织中的表达。结果：大鼠40周龄时，六味地黄丸治疗组bcl2 mRNA的表达水平为（1.25±0.07），较模型组（1.01±0.16）明显增高（Plt;0.01）；Bax mRNA的表达水平为（0.57±0.11），较模型组（1.18±0.28）有明显降低（Plt;0.01）。六味地黄丸治疗组的胰腺/体重比，较模型组增高，但差异无统计学意义。六味地黄丸治疗组的糖负荷能力明显高于模型组（Plt;0.05或Plt;0.01）。结论：六味地黄丸在转录水平可上调bcl2 mRNA的表达，下调Bax mRNA的表达，可能具有抗细胞凋亡的作用。 　　［关键词］nbsp; 糖尿病, 2型; 凋亡; 基因, bcl2; 基因, Bax; 六味地黄汤; 大鼠, OLETF 　　Effects of Liuwei Dihuang Pills on expressions of apoptosisrelated genes bcl2 and Bax in pancreas of OLETF rats 　　ABSTRACTnbsp; Objective: To investigate the effects of Liuwei Dihuang Pills (LWDHP) on expressions of apoptosisrelated genes bcl2 and Bax in pancreas of OLETF rats. Methods: Forty male OLETF rats were randomly divided into LWDHPtreated group and untreated group. Another ten male LETO rats were included in normal control group. OLETF rats in the LWDHPtreated group were given LWDHP (2.4 g#12539;kg1#12539;d1) orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. Body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerancenbsp; test (OGTT). Pancreas weights were recorded after rats were killed and the expression levels of bcl2 mRNA and Bax mRNA were detected by reverse transcriptasepolymerase chain reaction (RTPCR). Results: In the LWDHPtreated group, the expression of bcl2 mRNA in the pancreas of rats at the age of 40 weeks (1.25?.07) was much higher than that in the untreated group (1.01?.16), Plt;0.01. Bax mRNA level in the LWDHPtreated group (0.57?.11) was obviously lower than that in the untreate